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Winter wheat biomass was estimated using HJ CCD and MODIS data, combined with a radiation use efficiency model. Results were validated with ground measurement data. Winter wheat biomass estimated with HJ CCD data correlated well with observed biomass in different experiments (coefficients of determinationR2of 0.507, 0.556 and 0.499;n = 48). In addition,R2values between MODIS estimated and observed biomass are 0.420, 0.502 and 0.633. Even if we downscaled biomass estimated using HJ CCD data to MODIS pixel size (9 × 9 HJ CCD pixels to approximate that MODIS pixel),R2values between estimated and observed biomass were still higher than those from MODIS. We conclude that estimation with remote sensing data, such as the HJ CCD data with high spatial resolution and shorter revisit cycle, can show more detail in spatial pattern and improve the application of remote sensing on a local scale. There is also potential for applying the approach to many other studies, including agricultural production estimation, crop growth monitoring and agricultural ecosystem carbon cycle studies. [ABSTRACT FROM AUTHOR]