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Stable isotope analyses of oxygen are used in anthropology for such purposes as determination of origin of individuals, tracking migration routes or dynamics of human community relocation. The methodology related to oxygen isotope analysis has been founded on the relationship between its isotopic composition within phosphate groups of bone tissue (δ18Op) in individuals being analysed and the water consumed by such individuals (δ18Ow). Such a relationship has been observed in many species of mammals, including humans. However, the influence of culinary practices on the isotopic delta values of apatite phosphates of individuals has not yet been researched. The present study, which was conducted using laboratory rats, is an investigation of the influence of the thermal processing of water drank by such rats on the isotopic composition (δ18Op) of bone apatite. Increasing the value of the isotopic composition of water by about 6.1 ‰ during boiling resulted in an increase in the oxygen isotopic value δ18Op of rats drinking the water by about 4 ‰ (29%). It can be expected that regular consumption of heavily isotopic drinks and foods by humans may cause the δ18Op of individuals to exceed the range of isotopic environmental variability, even by a few per mille. [ABSTRACT FROM AUTHOR]