1. First structural characterization of Burkholderia vietnamiensis lipooligosaccharide from cystic fibrosis-associated lung transplantation strains.
- Author
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Ierano, Teresa, Silipo, Alba, Sturiale, Luisa, Garozzo, Domenico, Bryant, Clare, Lanzetta, Rosa, Parrilli, Michelangelo, Aldridge, Christine, Gould, F. Kate, Corris, Paul A., Khan, C. M. Anjam, De Soyza, Anthony, and Molinaro, Antonio
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BURKHOLDERIA , *OLIGOSACCHARIDES , *ENDOTOXINS , *CYSTIC fibrosis , *CELLS - Abstract
This is the first structural elucidation of the lipooligosaccharide (LOS) endotoxin isolated from Burkholderia vietnamiensis, a clinically important member of Burkholderia cepacia complex, a group of over 10 opportunistic species that are highly problematic in cystic fibrosis. We have characterized a novel LOS structure extracted from two clonal strains of B. vietnamiensis isolated from a cystic fibrosis patient who underwent lung transplantation. Strains were selected from the pretransplantation and post-transplantation periods and endotoxin was extracted. Subsequent analysis interestingly revealed identical oligosaccharidic sequences, but variation in lipid A moieties. Further, both LOS fractions were tested for their immunostimulatory activity on human myelomonocytic U937 cells and for signaling on an HEK293 cell line stably expressing both TLR 4 and MD-2. We observed an increase in lipid A acylation and a resultant increase in biological activity in bio-reporter assays of TNF-α secretion in the post-transplantation strain. [ABSTRACT FROM PUBLISHER]
- Published
- 2009
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