1. Characterization and Protection on Acute Liver Injury of a Polysaccharide MP-I from Mytilus Coruscus
- Author
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Tingting Guo, Binghua Jiao, Weihua Feng, Yang Li, Hongli Xu, Jianp eng Zhang, and Yi-feng Guo
- Subjects
Male ,Antioxidant ,medicine.medical_treatment ,Mice, Inbred Strains ,CCL4 ,Polysaccharide ,Biochemistry ,Antioxidants ,Mice ,chemistry.chemical_compound ,Polysaccharides ,medicine ,Animals ,Carbon Tetrachloride ,Glucans ,Glucan ,Mytilus ,chemistry.chemical_classification ,Liver injury ,biology ,Liver Diseases ,medicine.disease ,Malondialdehyde ,biology.organism_classification ,Liver ,chemistry ,Mytilus coruscus ,Carbon tetrachloride ,Chemical and Drug Induced Liver Injury - Abstract
In this study, we analyzed a water-soluble polysaccharide MP-I isolated from Mytilus coruscus. MP-I was obtained by hot-water extraction, anion-exchange and gel-permeation chromatography. Complete hydrolysis, periodate oxidation, methylation analysis, as well as Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) spectroscopy were conducted to elucidate its structure. MP-I was subjected to investigate the protective effect on carbon tetrachloride (CCl(4)) induced liver damage in male Kunming mice. Based on the data obtained, MP-I was found to be an alpha-(1--4)-D-glucan, branched with a single alpha-D-glucose at the C-6 position every eight residue, on average, along the main chain. Based on the calibration with Dextran, the glucan had a molecular weight of about 1.35 x 10(6) Da. Pharmacological studies revealed that MP-I could decrease serum alanine aminotransferase (ALT), serum aspartate aminotransferase (AST), and hepatic malondialdehyde aldehydes (MDA) levels, increase the hepatic total superoxide dismutase (T-SOD) activity, and improve hepatic damage in the CCl(4) induced liver injury in mice in a dose-dependent manner. The results suggest that the possible mechanism is due to its antioxidant activity of MP-I.
- Published
- 2007
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