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Single-difference (SD) ambiguity resolution (AR) and track-to-track (T2T) AR are two typical AR methods in precise orbit determination (POD) for Low Earth Orbit (LEO) satellites, which could improve the accuracy of orbits greatly. In this study, SD AR and T2T AR methods are introduced and analyzed. The performance of these two methods is assessed by three months of GPS observations from the Gravity Recovery and Climate Experiment Follow On (GRACE-FO) twin satellites. Results show that T2T AR is highly dependent on the stability of receiver hardware delays, while SD AR requires Fractional Cycle Bias (FCB) or Integer Recovery Clock (IRC) products. We find that these two methods have comparable performance in Reduced Dynamic Precise Orbit Determination (RDPOD), while SD AR slightly outperforms T2T AR in Kinematic Precise Orbit Determination (KPOD). We also find that SD AR has a higher AR success rate than T2T AR. Therefore, we recommend SD AR as the top choice in LEO orbit determination, and T2T AR can be a good alternative when FCB or IRC products are not available.