1. p38 MAPK signaling mediates IL-17-induced nitric oxide synthase expression in bone marrow cells
- Author
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Krstić, Aleksandra, Krstić, Aleksandra, Ilić, Vesna, Mojsilović, Slavko, Jovčić, Gordana, Milenković, Pavle B., Bugarski, Diana, Krstić, Aleksandra, Krstić, Aleksandra, Ilić, Vesna, Mojsilović, Slavko, Jovčić, Gordana, Milenković, Pavle B., and Bugarski, Diana
- Abstract
The effects of interleukin (IL)-17 on nitric oxide (NO) synthase (NOS) expression, as well as the participation of mitogen-activated protein kinases (MAPKs) in IL-17-mediated effects were examined in murine bone marrow cells. The results demonstrated the ability of IL-17 to upregulate the expression of mRNA for both inducible NOS and constitutive, endothelial NOS isoforms, as well as to enhance the phosphorylation of p38 MAPK. Moreover, both the NOS-inducing effect of IL-17 and the in vitro IL-17-mediated inhibition colony forming unit-erythroid (CFU-E) growth were dependent on p38 MAPK activity. The data demonstrating that the in vivo reducing effect of IL-17 on bone marrow CFU-E was prevented by co-treatment with the NOS inhibitor Nw-nitro-l-arginine methyl ester hydrochloride (L-NAME), implied that this effect is mediated through NOS activation. Besides revealing a link between the IL-17, NO, and haematopoiesis, data presented gave an insight into the mechanisms by which IL-17 exerts its modulatory effects on bone marrow cells.
- Published
- 2009