Search

【Objective】Discus fish (Symphysodon aequifasciatus) has special parental care behavior. In the study, prolactin (PRL) gene from discus fish was cloned and localized and its expression pattern during parental care was analyzed, with an aim to provide a basis for understanding the function of PRL in parental care of discus fish.【Method】A prolactin gene named dfprl was identified by analyzing the whole genome data of the discus fish through BLASTP. The CDS region of dfprl genes were used to design the primer of gene cloing and PCR. The full length of dfprl gene was cloned by RACE technology, and the structure of dfprl gene was analyzed by bioinformatics and its amino acid sequence was analyzed by physicochemical properties and phyletic evolution. Transcriptome sequencing was performed on brain, gonad and skin of discus fish at different rearing stages to explore the expression pattern of dfprl gene during parental care. The dfprl gene in the skin of discus fish was located by paraffin-fluorescence probe-FISH.【Result】The full length of dfprl was 1 282 bp, with 639 bp cDNA sequence encoding 212 amino acids, 309 bp 5'-UTR and 334 bp 3'-UTR. The dfprl protein has the typical domain of PRL family, the Hormone_1 domain. dfprl protein is highly similar to PRL protein of other Cichlidae, and the amino acid sequence homology of dfprl with Archocentrus centrarchus prolactin (AcPRL) was the highest (96.70%). The expression level of dfprl in gonad and skin increased gradually after the parent fish entered the rearing stage, and decreased at the end of the rearing stage. The paraffin-fluorescence probe-FISH results showed that dfprl was expressed in skin mucous cells, and it was coincident with the expression site of prolactin receptor (dfprlr) and was highly expressed in the early stage of parental care.【Conclusion】 The dfprl gene is highly conserved and has stable Hormone _1 domain. dfprl gene was highly expressed in the skin during the parental care stage, . and the expression sites of dfprl and dfprlr overlapped in skin mucous cells during the parental care stage of discus fish, suggesting that dfprl might be involved in the occurrence of unique parental care behavior of discus fish. [ABSTRACT FROM AUTHOR]

【Objective】Butyrophilin 1A1 (BTN1A1) gene from Symphysodon aequifasciatus was cloned and its express pattern under pathological infection was analyzed, to provide basis for better understanding of the function of BTN1A1 gene in S. aequifasciatu.【Method】The structure and evolutionary characteristics of two BTN1A1 genes (BTN1A1-1 and BTN1A1-2) were analyzed by bioinformatics analysis. Based on the transcriptome data of the skin of the S. aequifasciatu obtained at early stage, the CDS regions of BTN1A1-1 and BTN1A1-2 genes were used to design the primers for gene cloning. The expressions of BTN1A1-1 gene in various tissues and its expression pattern under the stimulus of Aeromonas hydrophila were analyzed by qRT-PCR.【Result】The ORF regions of BTN1A1-1 and BTN1A1-2 were 894 and 1 275 bp, encoding 298 and 424 amino acids, respectively. Both BTN1A1-1 and BTN1A1-2 had a classical structure of BTN, which contained a signal peptide, an Ig domain, an lg_like domain and a transmembrane domain. BTN1A1-2 also had a cytoplasmic domain. BTN1A1-1 showed a closer relationship with the genes found in cichlid fish. It shared the highest homology of 94.14% with the corresponding amino acid sequences of Archocentrus centrarchus; while BTN1A1-2 showed lower similarity with other types of fish, forming a special branch. The expressions of BTN1A1-1 and BTN1A1-2 were highly in gill, intestine and skin of S. aequifasciatu. The expression of BTN1A1-1 decreased significantly and then increased after the infection of A. hydrophila, while the expression of BTN1A1-2 increased significantly and then decreased.【Conclusion】The BTN1A1-1 gene was more evolutionarily conservative than BTN1A1-2. The differential expressions of BTN1A1-1 and BTN1A1-2 in response to immune stimulation suggests that they may be functionally differentiated in the immune defence of S. aequifasciatus. [ABSTRACT FROM AUTHOR]

Copyright of Guangdong Agricultural Sciences is the property of South China Agricultural University, Guangdong Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Guangdong Agricultural Sciences is the property of South China Agricultural University, Guangdong Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)