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Seed germination is a complex physiological and biochemical process that the storage material is broken into sucrose, glucose and other sugars compounds under the action of enzyme reaction in order to provide the carbon source and energy for seedling growth. In this study, the dynamics of storage carbohydrate mobilization, synthase activity and amylase were analyzed at different seed germination stages by using two different seeds that the grain nutrients are different are from different countries in sweet corn. The results showed that there were significant differences for storage carbohydrate mobilization between E22 and T26 including the mobilized seed reserve, seed reserve utilization, sucrose, glucose and fructose content. Starch is the key factor of seed germination in sweet corn because of the outstanding material utilization in T26. By comparison, the mobilized seed reserve in T26 was lower, while the seed reserve utilization efficiency was higher. During seed germination, sucrose and glucose contents were higher for E22 in 4-8 d and 6-10 d, while fructose content was higher for T26 in 10 d. Amylases and sucrose synthase activities were increased during the process of seed germination. Grey correlation analysis showed that the mobilized seed reserve was affected by the amylases during seed germination and seed reserve utilization was constrained by sucrose content, glucose content and fructose content. Improving of seed reserve utilization in sweet corn is of great significance to strengthen the seed germination and seedling growth, to enhance its competitiveness with weeds and to increase production. [ABSTRACT FROM AUTHOR]