1. Suppression of interferon-mediated anti-HBV response by single CpG methylation in the 5'-UTR of
- Author
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Keo-Heun, Lim, Eun-Sook, Park, Doo Hyun, Kim, Kyung Cho, Cho, Kwang Pyo, Kim, Yong Kwang, Park, Sung Hyun, Ahn, Seung Hwa, Park, Kee-Hwan, Kim, Chang Wook, Kim, Hong Seok, Kang, Ah Ram, Lee, Soree, Park, Heewoo, Sim, Juhee, Won, Kieun, Seok, Jueng Soo, You, Jeong-Hoon, Lee, Nam-Joon, Yi, Kwang-Woong, Lee, Kyung-Suk, Suh, Baik L, Seong, and Kyun-Hwan, Kim
- Subjects
Hepatitis B virus ,Proteome ,Down-Regulation ,Methylation ,Epigenesis, Genetic ,Minor Histocompatibility Antigens ,Repressor Proteins ,Tripartite Motif Proteins ,Mice ,Gene Expression Regulation ,Liver ,Hepatocytes ,Animals ,Humans ,CpG Islands ,Interferons ,5' Untranslated Regions ,Immune Evasion - Abstract
Interferons (IFNs) mediate direct antiviral activity. They play a crucial role in the early host immune response against viral infections. However, IFN therapy for HBV infection is less effective than for other viral infections.We explored the cellular targets of HBV in response to IFNs using proteome-wide screening.Using LC-MS/MS, we identified proteins downregulated and upregulated by IFN treatment in HBV X protein (HBx)-stable and control cells. We found several IFN-stimulated genes downregulated by HBx, includingWe verified our findings using a mouse model, primary human hepatocytes and human liver tissues. Our data elucidate a mechanism by which HBV evades the host innate immune system.
- Published
- 2016