1. Microbial bile salt hydrolases mediate the efficacy of faecal microbiota transplant in the treatment of recurrent
- Author
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Benjamin H, Mullish, Julie A K, McDonald, Alexandros, Pechlivanis, Jessica R, Allegretti, Dina, Kao, Grace F, Barker, Diya, Kapila, Elaine O, Petrof, Susan A, Joyce, Cormac G M, Gahan, Izabela, Glegola-Madejska, Horace R T, Williams, Elaine, Holmes, Thomas B, Clarke, Mark R, Thursz, and Julian R, Marchesi
- Subjects
DNA, Bacterial ,Clostridioides difficile ,Fecal Microbiota Transplantation ,Amidohydrolases ,Gastrointestinal Microbiome ,Mice, Inbred C57BL ,Disease Models, Animal ,Mice ,Recurrence ,Tandem Mass Spectrometry ,Clostridium Infections ,Animals ,Humans ,Female ,Glycocholic Acid - Abstract
Faecal microbiota transplant (FMT) effectively treats recurrentUsing stool collected from patients and donors pre-FMT/post-FMT for rCDI, we performed 16S rRNA gene sequencing, ultra performance liquid chromatography mass spectrometry (UPLC-MS) bile acid profiling, BSH activity measurement, and qPCR ofFrom metataxonomics, pre-FMT stool demonstrated a reduced proportion of BSH-producing bacterial species compared with donors/post-FMT. Pre-FMT stool was enriched in taurocholic acid (TCA, a potentRestoration of gut BSH functionality contributes to the efficacy of FMT in treating rCDI.
- Published
- 2018