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Start Over Author "Domenico, Mavilio" Journal haematologica
5 results on '"Domenico, Mavilio"'

1. Single-cell profiling reveals the dynamics of cytomegalovirus-specific T cells in haploidentical hematopoietic stem cell transplantation

Author

Jasper J. P. van Beek, Simone Puccio, Alessandra Roberto, Federica De Paoli, Giulia Graziano, Elisa Salviato, Giorgia Alvisi, Veronica Zanon, Alice Scarpa, Elisa Zaghi, Michela Calvi, Clara Di Vito, Rossana Mineri, Barbara Sarina, Chiara De Philippis, Armando Santoro, Jacopo Mariotti, Stefania Bramanti, Francesco Ferrari, Luca Castagna, Domenico Mavilio, and Enrico Lugli

Subjects
Diseases of the blood and blood-forming organs, RC633-647.5
Published
2021
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2. The early expansion of anergic NKG2Apos/CD56dim/CD16neg natural killer represents a therapeutic target in haploidentical hematopoietic stem cell transplantation

Author

Alessandra Roberto, Clara Di Vito, Elisa Zaghi, Emilia Maria Cristina Mazza, Arianna Capucetti, Michela Calvi, Paolo Tentorio, Veronica Zanon, Barbara Sarina, Jacopo Mariotti, Stefania Bramanti, Elena Tenedini, Enrico Tagliafico, Silvio Bicciato, Armando Santoro, Mario Roederer, Emanuela Marcenaro, Luca Castagna, Enrico Lugli, and Domenico Mavilio

Subjects
Diseases of the blood and blood-forming organs, RC633-647.5
Abstract

Natural killer cells are the first lymphocyte population to reconstitute early after non-myeloablative and T cell-replete haploidentical hematopoietic stem cell transplantation with post-transplant infusion of cyclophosphamide. The study herein characterizes the transient and predominant expansion starting from the second week following haploidentical hematopoietic stem cell transplantation of a donor-derived unconventional subset of NKp46neg-low/CD56dim/CD16neg natural killer cells expressing remarkably high levels of CD94/NKG2A. Both transcription and phenotypic profiles indicated that unconventional NKp46neg-low/CD56dim/CD16neg cells are a distinct natural killer cell subpopulation with features of late stage differentiation, yet retaining proliferative capability and functional plasticity to generate conventional NKp46pos/CD56bright/CD16neg-low cells in response to interleukin-15 plus interleukin-18. While present at low frequency in healthy donors, unconventional NKp46neg-low/CD56dim/CD16neg cells are greatly expanded in the seven weeks following haploidentical hematopoietic stem cell transplantation, and express high levels of the activating receptors NKG2D and NKp30 as well as of the lytic granules Granzyme-B and Perforin. Nonetheless, NKp46neg-low/CD56dim/CD16neg cells displayed a markedly defective cytotoxicity that could be reversed by blocking the inhibitory receptor CD94/NKG2A. These data open new and important perspectives to better understand the ontogenesis/homeostasis of human natural killer cells and to develop a novel immune-therapeutic approach that targets the inhibitory NKG2A check-point, thus unleashing natural killer cell alloreactivity early after haploidentical hematopoietic stem cell transplantation.

Published
2018
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3. The early expansion of anergic NKG2Apos/CD56dim/CD16neg natural killer represents a therapeutic target in haploidentical hematopoietic stem cell transplantation

Author

Emilia Maria Cristina Mazza, Elena Tenedini, Stefania Bramanti, Barbara Sarina, Luca Castagna, Jacopo Mariotti, Domenico Mavilio, Clara Di Vito, Paolo Tentorio, Enrico Tagliafico, Veronica Zanon, Silvio Bicciato, Emanuela Marcenaro, Elisa Zaghi, Enrico Lugli, Arianna Capucetti, Alessandra Roberto, Armando Santoro, Michela Calvi, and Mario Roederer

Subjects
0301 basic medicine, Lymphocyte, medicine.medical_treatment, Population, Hematopoietic stem cell transplantation, Biology, Immunophenotyping, haploidentical HSCT, Natural killer cell, 03 medical and health sciences, 0302 clinical medicine, medicine, Lymphocytes, Receptor, education, education.field_of_study, Clonal anergy, Hematology, NKG2D, Cell Therapy and Immunotherapy, Transplantation, 030104 developmental biology, medicine.anatomical_structure, NK cell immune-reconstitution, Cancer research, Cell Therapy and Immunotherapy, Immunophenotyping, Lymphocytes, NK cell immune-reconstitution, haploidentical HSCT, 030215 immunology
Abstract

Natural killer cells are the first lymphocyte population to reconstitute early after non-myeloablative and T cell-replete haploidentical hematopoietic stem cell transplantation with post-transplant infusion of cyclophosphamide. The study herein characterizes the transient and predominant expansion starting from the second week following haploidentical hematopoietic stem cell transplantation of a donor-derived unconventional subset of NKp46neg-low/CD56dim/CD16neg natural killer cells expressing remarkably high levels of CD94/NKG2A. Both transcription and phenotypic profiles indicated that unconventional NKp46neg-low/CD56dim/CD16neg cells are a distinct natural killer cell subpopulation with features of late stage differentiation, yet retaining proliferative capability and functional plasticity to generate conventional NKp46pos/CD56bright/CD16neg-low cells in response to interleukin-15 plus interleukin-18. While present at low frequency in healthy donors, unconventional NKp46neg-low/CD56dim/CD16neg cells are greatly expanded in the seven weeks following haploidentical hematopoietic stem cell transplantation, and express high levels of the activating receptors NKG2D and NKp30 as well as of the lytic granules Granzyme-B and Perforin. Nonetheless, NKp46neg-low/CD56dim/CD16neg cells displayed a markedly defective cytotoxicity that could be reversed by blocking the inhibitory receptor CD94/NKG2A. These data open new and important perspectives to better understand the ontogenesis/homeostasis of human natural killer cells and to develop a novel immune-therapeutic approach that targets the inhibitory NKG2A check-point, thus unleashing natural killer cell alloreactivity early after haploidentical hematopoietic stem cell transplantation.

Published
2018

4. The early expansion of anergic NKG2A

Author

Alessandra, Roberto, Clara, Di Vito, Elisa, Zaghi, Emilia Maria Cristina, Mazza, Arianna, Capucetti, Michela, Calvi, Paolo, Tentorio, Veronica, Zanon, Barbara, Sarina, Jacopo, Mariotti, Stefania, Bramanti, Elena, Tenedini, Enrico, Tagliafico, Silvio, Bicciato, Armando, Santoro, Mario, Roederer, Emanuela, Marcenaro, Luca, Castagna, Enrico, Lugli, and Domenico, Mavilio

Subjects
Clonal Anergy, Transplantation Conditioning, Receptors, IgG, Hematopoietic Stem Cell Transplantation, GPI-Linked Proteins, CD56 Antigen, Lymphocyte Subsets, Article, Killer Cells, Natural, NK Cell Lectin-Like Receptor Subfamily K, Cell Therapy & Immunotherapy, Hematologic Neoplasms, Transplantation, Haploidentical, Humans, Immunotherapy, NK Cell Lectin-Like Receptor Subfamily C, Cells, Cultured, Cell Proliferation
Abstract

Natural killer cells are the first lymphocyte population to reconstitute early after non-myeloablative and T cell-replete haploidentical hematopoietic stem cell transplantation with post-transplant infusion of cyclophosphamide. The study herein characterizes the transient and predominant expansion starting from the second week following haploidentical hematopoietic stem cell transplantation of a donor-derived unconventional subset of NKp46neg-low/CD56dim/CD16neg natural killer cells expressing remarkably high levels of CD94/NKG2A. Both transcription and phenotypic profiles indicated that unconventional NKp46neg-low/CD56dim/CD16neg cells are a distinct natural killer cell subpopulation with features of late stage differentiation, yet retaining proliferative capability and functional plasticity to generate conventional NKp46pos/CD56bright/CD16neg-low cells in response to interleukin-15 plus interleukin-18. While present at low frequency in healthy donors, unconventional NKp46neg-low/CD56dim/CD16neg cells are greatly expanded in the seven weeks following haploidentical hematopoietic stem cell transplantation, and express high levels of the activating receptors NKG2D and NKp30 as well as of the lytic granules Granzyme-B and Perforin. Nonetheless, NKp46neg-low/CD56dim/CD16neg cells displayed a markedly defective cytotoxicity that could be reversed by blocking the inhibitory receptor CD94/NKG2A. These data open new and important perspectives to better understand the ontogenesis/homeostasis of human natural killer cells and to develop a novel immune-therapeutic approach that targets the inhibitory NKG2A check-point, thus unleashing natural killer cell alloreactivity early after haploidentical hematopoietic stem cell transplantation.

Published
2017

5. Single-cell profiling reveals the dynamics of cytomegalovirus-specific T cells in haploidentical hematopoietic stem cell transplantation

Author

Rossana Mineri, Clara Di Vito, Simone Puccio, Federica De Paoli, Alessandra Roberto, Armando Santoro, Jasper J. P. van Beek, Giorgia Alvisi, Elisa Zaghi, Michela Calvi, Chiara De Philippis, Elisa Salviato, Veronica Zanon, Francesco Ferrari, Jacopo Mariotti, Domenico Mavilio, Luca Castagna, Stefania Bramanti, Barbara Sarina, Enrico Lugli, Alice Scarpa, and Giulia Graziano

Subjects
0303 health sciences, Transplantation Conditioning, business.industry, T-Lymphocytes, medicine.medical_treatment, Cell, Hematopoietic Stem Cell Transplantation, Congenital cytomegalovirus infection, Cytomegalovirus, Graft vs Host Disease, Hematology, Hematopoietic stem cell transplantation, medicine.disease, 03 medical and health sciences, 0302 clinical medicine, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Cytomegalovirus Infections, medicine, Cancer research, Humans, Letters to the Editor, business, 030304 developmental biology
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