Lum, Wai Mun, Benico, Garry, Doan-Nhu, Hai, Furio, Elsa, Leaw, Chui Pin, Leong, Sandric Chee Yew, Lim, Po Teen, Lim, Weol Ae, Lirdwitayaprasit, Thaithaworn, Lu, Songhui, Muawanah, Nguyen, Nguyen Van, Orlova, Tatiana Yu., Rachman, Arief, Sakamoto, Setsuko, Takahashi, Kazuya, Teng, Sing Tung, Thoha, Hikmah, Wang, Pengbin, and Yñiguez, Aletta T.
• Aside from East Asia, historical records also showed the wide distribution of harmful raphidophyte Chattonella in Southeast Asian coastal waters. • Only one genotype, Chattonella marina complex, has been observed in East Asia. • Two Chattonella genotypes, C. marina complex and C. subsalsa , have occurred in Southeast Asia and were associated with fisheries damage. • Oboe-shaped mucocyst was observed from the cultures of C. marina from Indonesia, Malaysia, Japan and Russia for the first time. Red tides and associated fisheries damage caused by the harmful raphidophyte Chattonella were reassessed based on the documented local records for 50 years to understand the distribution and economic impacts of the harmful species in the Western Pacific. Blooms of Chattonella with fisheries damage have been recorded in East Asia since 1969, whereas they have been only recorded in Southeast Asia since the 1980s. Occurrences of Chattonella have been documented from six Southeast Asian countries, Indonesia, Malaysia, Philippines, Singapore, Thailand and Viet Nam, with mass mortalities mainly of farmed shrimp in 1980–1990s, and farmed fish in 2000–2010s. These occurrences have been reported with the names of C. antiqua, C. marina, C. ovata, C. subsalsa and Chattonella sp., owing to the difficulty of microscopic species identification, and many were not supported with molecular data. To determine the distribution of C. marina complex and C. subsalsa in Southeast Asia, molecular phylogeny and microscopic observation were also carried out for cultures obtained from Indonesia, Malaysia, Japan, Philippines, Russia, Singapore and Thailand. The results revealed that only the genotype of C. marina complex has been detected from East Asia (China, Japan, Korea and Russia), whereas both C. marina complex (Indonesia and Malaysia) and C. subsalsa (Philippines, Singapore and Thailand) were found in Southeast Asia. Ejection of mucocysts has been recognized as a diagnostic character of C. subsalsa , but it was also observed in our cultures of C. marina isolated from Indonesia, Malaysia, Japan, and Russia. Meanwhile, the co-occurrences of the two harmful Chattonella species in Southeast Asia, which are difficult to distinguish solely based on their morphology, suggest the importance of molecular identification of Chattonella genotypes for further understanding of their distribution and negative impacts. [ABSTRACT FROM AUTHOR]