1. Study of hrpNCSDS001 and the gene expression profile of Arabidopsis thaliana induced by HarpinCSDS001
- Author
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Yu-Bi Huang, Mao-Lin Cao, Jue Zhang, and Bo-Ji Wu
- Subjects
Sequence analysis ,Molecular Sequence Data ,Arabidopsis ,Biology ,Erwinia ,Gene Expression Regulation, Plant ,Gene expression ,Botany ,Escherichia coli ,Arabidopsis thaliana ,MYB ,Amino Acid Sequence ,Cloning, Molecular ,Gene ,Oligonucleotide Array Sequence Analysis ,Regulation of gene expression ,Gene Expression Profiling ,food and beverages ,Sequence Analysis, DNA ,General Medicine ,biology.organism_classification ,Molecular biology ,WRKY protein domain ,Kinetics ,Pectobacterium carotovorum ,Bacterial Outer Membrane Proteins - Abstract
Erwinia carotovora subsp. carotovora CSDS001 elicits hypersensitive reaction (HR) in tobacco. From the genomic libraries of Erwinia carotovora subsp. carotovora CSDS001, the hrpNCSDS001 gene (GenBank number AY939927), was isolated. The hrpNCSDS001 fusion protein was produced in Escherichia coli, and was used to induce HR by injecting into tobacco. We further examined the global regulation of Arabidopsis thaliana genes in response to HarpinCSDS001 at a concentration of 30 miccrog/mL. We indicated that 912, 1787, 2393, 1833 and 1,755 genes that were regulated significantly (log ratioor=-1 oror=1) at 3 h, 12 h, 24 h, 36 h and 48 h respectively after the treatment. Analysis of some transcription factors (TF) showed that 13 TF families responded to HarpinCSDS001 including ZIM, BES1, TCP, C2C2, AP2/EREBP, WRKY, bHLH, bZIP, GARP, MYB, NAC, HB, C2H2. These families mainly function in biological processes of plant defense, pho-tosynthesisdevelopment and flowering.
- Published
- 2007
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