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Tibetan Buddhism has an inseparable connection with the spatial characteristics, development process, and human-natural environment of the Tibetan Plateau. This paper takes monasteries as carriers of Tibetan Buddhist culture in U-Tsang region which is one of the traditional geographical units of Tibet. Using geospatial analysis methods, this study explores the spatial quantification characteristics of Tibetan Buddhist monasteries and qualitatively and quantitatively analyzes the factors that influence the spatial distribution of monasteries. The results indicate that: (1) Political activities of human society influence the pattern of political power. Under the unique context of Tibetan theocracy system, the scope of political authority has a significant influence and affects the religious spatial pattern in the U-Tsang region throughout history. The distribution of monasteries in the U-Tsang region shows significant spatial differences at three sub-regional scales. (2) The religious spatial pattern in the U-Tsang is the result of the diverse interaction of human-natural factors. The results indirectly endorse that religious space is an inevitable product of the interaction between humans and the environment. (3) The religious spatial distribution patterns in the three major Tibetan regions have distinct characteristics, closely related to the superior conditions of their respective historical and geographical environments. [ABSTRACT FROM AUTHOR]