7 results on '"Wang, Yikui"'
Search Results
2. Mapping and validation of the epistatic D and P genes controlling anthocyanin biosynthesis in the peel of eggplant (Solanum melongena L.) fruit.
- Author
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You, Qian, Li, Huimin, Wu, Jun, Li, Tao, Wang, Yikui, Sun, Guangwen, Li, Zhiliang, and Sun, Baojuan
- Subjects
EGGPLANT ,ANTHOCYANINS ,BIOSYNTHESIS ,FRUIT skins ,FRUIT ,DOMINANCE (Genetics) - Abstract
Fruit color is an important trait influencing the commercial value of eggplant fruits. Three dominant genes (D , P and Y) cooperatively control the anthocyanin coloration in eggplant fruits, but none has been mapped. In this study, two white-fruit accessions (19 141 and 19 147) and their F
2 progeny, with 9:7 segregation ratio of anthocyanin pigmented versus non-pigmented fruits, were used for mapping the D and P genes. A high-density genetic map was constructed with 5270 SNPs spanning 1997.98 cM. Three QTLs were identified, including two genes on chromosome 8 and one on chromosome 10. Gene expression analyses suggested that the SmANS on chromosome 8 and SmMYB1 on chromosome 10 were the putative candidate genes for P and D , respectively. We further identified (1) a SNP leading to a premature stop codon within the conserved PLN03176 domain of SmANS in 19 141, (2) a G base InDel in the promoter region leading to an additional cis -regulatory element and (3) a 6-bp InDel within the R2-MYB DNA binding domain of SmMYB1 , in 19 147. Subsequently, these three variations were validated by PARMS technology as related to phenotypes in the F2 population. Moreover, silencing of SmANS or SmMYB1 in the purple red fruits of F1 (E3316) led to inhibition of anthocyanin biosynthesis in the peels. Conversely, overexpression of SmANS or SmMYB1 restored anthocyanin biosynthesis in the calli of 19 141 and 19 147 respectively. Our findings demonstrated the epistatic interactions underlying the white color of eggplant fruits, which can be potentially applied to breeding of eggplant fruit peel color. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
3. A QTL of eggplant shapes the rhizosphere bacterial community, co-responsible for resistance to bacterial wilt
- Author
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Gong, Chao, Wang, Zhenshuo, Li, Zhiliang, Sun, Baojuan, Luo, Wenlong, Luo, Shanwei, Chen, Shuting, Mai, Peiting, Li, Zhenxing, Li, Ye, Wang, Yikui, and Li, Tao
- Abstract
Resistant crop cultivars can recruit beneficial rhizobacteria to resist disease. However, whether this recruitment is regulated by quantitative trait loci (QTL) is unclear. The role of QTL in recruiting specific bacteria against bacterial wilt (BW) is an important question of practical significance to disease management. Here, to identify QTL controlling BW resistance, Super-BSA was performed in F2plants derived from resistant eggplant cultivar R06112 × susceptible cultivar S55193. The QTL was narrowed down through BC1F1-BC3F1individuals by wilting symptoms and KASP markers. Rhizosphere bacterial composition of R06112, S55193, and resistant individuals EB158 (with the QTL) and susceptible individuals EB327 (without QTL) from BC2F1generation were assessed by Illumina sequencing-based analysis, and the activation of plant immunity by the bacterial isolates was analyzed. Evidence showed that BW-resistant is controlled by one QTL located at the 270 kb region on chromosome 10, namely EBWR10, and nsLTPsas candidate genes confirmed by RNA-Seq. EBWR10has a significant effect on rhizobacteria composition and significantly recruits Bacillus. pp. A SynCom of three isolated Bacillus. pp trains significantly reduced the disease incidence, changed activities of CAT, PPO, and PAL and concentration of NO, H2O2, and O2−, activated SA and JA signaling-dependent ISR, and displayed immune activation against Ralstonia solanacearumin eggplant. Our findings demonstrate for the first time that the QTL can recruit beneficial rhizobacteria, which jointly promote the suppression of BW. This method charts a path to develop the QTL in resistant cultivar-driven probiotics to ameliorate plant diseases.
- Published
- 2024
- Full Text
- View/download PDF
4. Gibberellins involved in fruit ripening and softening by mediating multiple hormonal signals in tomato
- Author
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Wu, Mengbo, Liu, Kaidong, Li, Honghai, Li, Ying, Zhu, Yunqi, Su, Dan, Zhang, Yaoxin, Deng, Heng, Wang, Yikui, and Liu, Mingchun
- Abstract
The phytohormone ethylene is well known for its important role in the ripening of climacteric fruit, such as tomato (Solanum lycopersicum). However, the role and mode of action of other plant hormones in climacteric fruit ripening regulation are not fully understood. Here, we showed that exogenous GA treatment or increasing endogenous gibberellin content by overexpressing the gibberellin synthesis gene SlGA3ox2specifically in fruit tissues delayed tomato fruit ripening, whereas treatment with the GA biosynthesis inhibitor paclobutrazol (PAC) accelerated fruit ripening. Moreover, exogenous ethylene treatment cannot completely reverse the delayed fruit ripening phenotype. Furthermore, exogenous GA treatment of ethylene signalling mutant Never ripe(Nr) or SlEBF3-overexpressing lines still delayed fruit ripening, suggesting that GA involved in fruit ripening partially depends on ethylene. Transcriptome profiling showed that gibberellin affect the ripening of fruits by modulating the metabolism and signal transduction of multiple plant hormones, such as auxin and abscisic acid, in addition to ethylene. Overall, the results of this study provide new insight into the regulation of gibberellin in fruit ripening through mediating multiple hormone signals.
- Published
- 2024
- Full Text
- View/download PDF
5. Mapping and validation of the epistatic Dand Pgenes controlling anthocyanin biosynthesis in the peel of eggplant (Solanum melongenaL.) fruit
- Author
-
You, Qian, Li, Huimin, Wu, Jun, Li, Tao, Wang, Yikui, Sun, Guangwen, Li, Zhiliang, and Sun, Baojuan
- Abstract
Fruit color is an important trait influencing the commercial value of eggplant fruits. Three dominant genes (D, Pand Y) cooperatively control the anthocyanin coloration in eggplant fruits, but none has been mapped. In this study, two white-fruit accessions (19 141 and 19 147) and their F2progeny, with 9:7 segregation ratio of anthocyanin pigmented versus non-pigmented fruits, were used for mapping the Dand Pgenes. A high-density genetic map was constructed with 5270 SNPs spanning 1997.98 cM. Three QTLs were identified, including two genes on chromosome 8 and one on chromosome 10. Gene expression analyses suggested that the SmANSon chromosome 8 and SmMYB1on chromosome 10 were the putative candidate genes for Pand D, respectively. We further identified (1) a SNP leading to a premature stop codon within the conserved PLN03176 domain of SmANSin 19 141, (2) a G base InDel in the promoter region leading to an additional cis-regulatory element and (3) a 6-bp InDel within the R2-MYB DNA binding domain of SmMYB1, in 19 147. Subsequently, these three variations were validated by PARMS technology as related to phenotypes in the F2population. Moreover, silencing of SmANSor SmMYB1in the purple red fruits of F1(E3316) led to inhibition of anthocyanin biosynthesis in the peels. Conversely, overexpression of SmANSor SmMYB1restored anthocyanin biosynthesis in the calli of 19 141 and 19 147 respectively. Our findings demonstrated the epistatic interactions underlying the white color of eggplant fruits, which can be potentially applied to breeding of eggplant fruit peel color.
- Published
- 2023
- Full Text
- View/download PDF
6. A QTL of eggplant shapes the rhizosphere bacterial community, co-responsible for resistance to bacterial wilt.
- Author
-
Gong C, Wang Z, Li Z, Sun B, Luo W, Luo S, Chen S, Mai P, Li Z, Li Y, Wang Y, and Li T
- Abstract
Resistant crop cultivars can recruit beneficial rhizobacteria to resist disease. However, whether this recruitment is regulated by quantitative trait loci (QTL) is unclear. The role of QTL in recruiting specific bacteria against bacterial wilt (BW) is an important question of practical significance to disease management. Here, to identify QTL controlling BW resistance, Super-BSA was performed in F
2 plants derived from resistant eggplant cultivar R06112 × susceptible cultivar S55193. The QTL was narrowed down through BC1 F1 -BC3 F1 individuals by wilting symptoms and KASP markers. Rhizosphere bacterial composition of R06112, S55193, and resistant individuals EB158 (with the QTL) and susceptible individuals EB327 (without QTL) from BC2 F1 generation were assessed by Illumina sequencing-based analysis, and the activation of plant immunity by the bacterial isolates was analyzed. Evidence showed that BW-resistant is controlled by one QTL located at the 270 kb region on chromosome 10, namely EBWR10 , and nsLTPs as candidate genes confirmed by RNA-Seq. EBWR10 has a significant effect on rhizobacteria composition and significantly recruits Bacillus . pp. A SynCom of three isolated Bacillus . pp trains significantly reduced the disease incidence, changed activities of CAT, PPO, and PAL and concentration of NO, H2 O2 , and O2 - , activated SA and JA signaling-dependent ISR, and displayed immune activation against Ralstonia solanacearum in eggplant. Our findings demonstrate for the first time that the QTL can recruit beneficial rhizobacteria, which jointly promote the suppression of BW. This method charts a path to develop the QTL in resistant cultivar-driven probiotics to ameliorate plant diseases., Competing Interests: The authors declare that there are no conflicts of interest., (© The Author(s) 2024. Published by Oxford University Press on behalf of Nanjing Agricultural University.)- Published
- 2023
- Full Text
- View/download PDF
7. Gibberellins involved in fruit ripening and softening by mediating multiple hormonal signals in tomato.
- Author
-
Wu M, Liu K, Li H, Li Y, Zhu Y, Su D, Zhang Y, Deng H, Wang Y, and Liu M
- Abstract
The phytohormone ethylene is well known for its important role in the ripening of climacteric fruit, such as tomato ( Solanum lycopersicum ). However, the role and mode of action of other plant hormones in climacteric fruit ripening regulation are not fully understood. Here, we showed that exogenous GA treatment or increasing endogenous gibberellin content by overexpressing the gibberellin synthesis gene SlGA3ox2 specifically in fruit tissues delayed tomato fruit ripening, whereas treatment with the GA biosynthesis inhibitor paclobutrazol (PAC) accelerated fruit ripening. Moreover, exogenous ethylene treatment cannot completely reverse the delayed fruit ripening phenotype. Furthermore, exogenous GA treatment of ethylene signalling mutant Never ripe ( Nr ) or SlEBF3 -overexpressing lines still delayed fruit ripening, suggesting that GA involved in fruit ripening partially depends on ethylene. Transcriptome profiling showed that gibberellin affect the ripening of fruits by modulating the metabolism and signal transduction of multiple plant hormones, such as auxin and abscisic acid, in addition to ethylene. Overall, the results of this study provide new insight into the regulation of gibberellin in fruit ripening through mediating multiple hormone signals., Competing Interests: The authors declare no conflict of interest., (© The Author(s) 2024. Published by Oxford University Press on behalf of Nanjing Agricultural University.)
- Published
- 2023
- Full Text
- View/download PDF
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