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Spine grape (Vitis davidii Foëx), an important wild grape species in South China, has gained attention because of its health-promoting effects and use in the wine industry. Fruit quality plays an important role in determining the quality of wine; however, a suitable evaluation system to monitor its fruit quality has not been established. The fruit quality characteristics (phenolics and aromas) of 15 spine grapes grown in China were evaluated using a combination of principal component and cluster analyses. The total sugar, organic acid, and phenolic content ranged from 81.80 to 154.89 mg·g−1, 8.02 to 15.48 mg·g−1, and 5.58 to 20.12 mg·g−1, respectively. The comprehensive assessment by principal component analysis revealed that ‘Red xiangzhenzhu’ had the highest quality and ‘Hongjiangci10’ and ‘Ziluolan’ the lowest quality. Cluster analysis using k-means grouped the cultivars into three clusters based on their quality: Cluster 1 grouped those with inferior quality (‘Hongjiangci09’, ‘Hongjiangci10’, ‘Hongjiangci11’, and ‘Hongjiangci07’, etc.), Cluster2 grouped those with average quality (‘Ciputao3#,’ ‘Ziluolan’, and ‘Xiangci4#’), and Cluster3 grouped those with superior quality (‘Red xiangzhenzhu’ and ‘Green xiangzhenzhu’). A combination of principal component analysis and cluster analysis provides a comprehensive and objective evaluation system for determining the quality of grape cultivars. This study is important for the systematic evaluation and utilization of spine grape resources.

In this study, we measured the anthocyanin composition and content in the 'Kyoho' grape cultivar and its derivatives via ultra-performance liquid chromatography-mass spectrometry and characterized the MybA-related genes at the color locus via capillary electrophoresis and quantitative real-time polymerase chain reaction. A total of 30 anthocyanins (15 monoglucoside and 15 diglucoside) were detected. Peonidin-3-O-(t-6"-O-coumaroyl)-glucoside-5-O-glucoside was the most abundant component, and the content of malvidin-3-O-(c-6"-O-coumaroyl)-glucoside-5-O-glucoside was low in all cultivars. All 49 cultivars contained VvmybA1, VvmybA2, and VvmybA3, whereas only the black-skinned cultivars contained VlmybA2. The anthocyanin content in the cultivars that contained VlmybA2 was significantly higher than other cultivars. These results could provide information for future color breeding programs in grapes. [ABSTRACT FROM AUTHOR]

In this study, we present the molecular characterization of 61 Chinese grape landraces and 33 foreign cultivars by using nine microsatellite DNA markers. A total of 115 distinct alleles were amplified, and the average allele number was 12.78. The average observed and expected heterozygosity values were 0.797 and 0.839, respectively. The effective allele numbers ranged from 5.011 to 8.575. The average polymorphism information content (PIC) was 0.816. Eighty distinct genotypes were detected, and new synonyms and homonyms were found. The clustering dendrogram indicated that 94 Vitis materials could be divided into five major groups, and the cluster analysis showed that part of the Chinese grape landraces had a close relationship with the foreign cultivars. Assessment of the true cultivar identity, and the identification of synonyms and homonyms will be a contribution to improve the grape germplasm management and protect breeders' intellectual rights. [ABSTRACT FROM AUTHOR]