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5 results on '"Kumiko Tsuboi"'

1. Functional analysis of three novel cell lines derived from human papillary thyroid carcinomas with three different clinical courses

Author

Kumiko Tsuboi, Mayumi Ishikawa, Toshihide Ito, Kazutoshi Shibuya, Shiro Minami, Hisashi Hashimoto, Toshiaki Tachibana, Gen Yoshino, Takahisa Hirose, and Junko Toyomura

Subjects
Adult, Male, Cancer Research, medicine.medical_specialty, Adolescent, endocrine system diseases, medicine.medical_treatment, Cell, Thyrotropin, Thyroglobulin, Metastasis, Thyroid carcinoma, Cell Line, Tumor, Internal medicine, medicine, Humans, Thyroid Neoplasms, Insulin-Like Growth Factor I, Neoplasm Metastasis, Thyroid cancer, Aged, 80 and over, Human Growth Hormone, Cell growth, business.industry, Carcinoma, Cell Biology, medicine.disease, Carcinoma, Papillary, Thyroxine, Cell Transformation, Neoplastic, Phenotype, Endocrinology, medicine.anatomical_structure, Thyroid Cancer, Papillary, Cervical lymph nodes, Lymphatic Metastasis, Cancer research, Female, Stem cell, Lysosomes, business
Abstract

Papillary thyroid carcinoma (PTC) is the most frequent thyroid carcinoma. PTC cell lines have been of considerable value in studying aspects of thyroid cancer, such as gene expression, cell proliferation, and differentiation. Here we report three novel PTC lines established from three patients with different backgrounds. Case 1 was a 38-year-old woman with PTC in the right thyroid lobe, with no metastasis. The cell line was established from the resection sample and named D-PTC. The cell line consisted of epithelial cells with few lysosomes and showed a pavement structure and follicular formation at confluency. There was a little pilling up. The secretion of free thyroxin (fT4) and thyroglobulin (Tg) was increased by TSH, or GH and IGF-I treatment. Case 2 was a 22-year-old woman with PTC initially in the right thyroid lobe, but 4 years after the right lobe resection, PTC metastasis was observed in left lobe. The cell line was established from a sample of the second resection and named UD-PTC. This cell line consisted of small epithelial cells with evident lysosomes and exhibited floating cell clusters. The secretion of fT4 and Tg was slightly increased by TSH, or GH and IGF-I treatment. Case 3 was an 85-year-old man with PTC and with acromegaly. Metastasis was observed at cervical lymph nodes. The cell line was derived from the metastasis region and named A-PTC. This cell line consisted of small epithelial cells and many lysosomes. The cells frequently showed pilling up. The secretion of fT4 and Tg was significantly increased by GH and IGF-I treatment. We have established three PTC cell lines with substantial variation in their phenotype. The cell lines may be useful for thyroid cancer research.

Published
2014
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2. Proceedings of The 28th Annual Meeting of the Japan Human Cell Society 23 August 2009, Tsukuba, Ibaraki, Japan

Author

Osamu Nunobiki, Masanori Fujita, Yoshinobu Kubota, Yoshitaka Watanabe, Yasuyuki Araki, Takuya Kawaguchi, Sadamu Noda, Gen Yoshino, Yosuke Kameoka, Shuji Seki, Li Qiang, Kiyotaka Yokogami, Michio Kosugi, Koichi Nemoto, Noriko Tominaga, Kumiko Katagiri, Akiyoshi Nakayama, Shinya Suzuki, Yutaka Horiguchi, Mayumi Ishikawa, Makoto Yasuda, Jun Nakashima, Inaho Danjoh, Takeshi Hashimoto, Toshitaka Seno, Yoshiaki Okamoto, Minako Suzuki, Takuya Sato, Yuko Tokieda, Hideo Takeshima, Yuji Morimoto, Hiroaki Kataoka, Masatsugu Ueda, Takeo Kosaka, Suguru Shirotake, Akira Miyajima, Hiroshi Ishikawa, Keiji Kawamoto, Kiyo Torii, Takumi Era, Michiko Yamamoto, Naomi Sato, Manabu Kinoshita, Yasuhisa Araki, Tomoharu Tamagawa, Mototsugu Oya, Eisaku Toji, Hirotaka Matsuo, Eriko Suzuki, Norihiro Kuwahata, Takahiro Yamahara, Toshiaki Tachibana, Tomomi Nakagawa, Yi Li, Miyuki Kawakami, Masaaki Tachibana, Eiji Kikuchi, Shinji Izuma, Tsuyoshi Fukushima, Yoshihiro Numa, Koji Eto, Megumi Iguchi, Tohru Masui, Yasuo Yoshihara, Toshihiro Akaike, Nariyoshi Shinomiya, Isamu Ishiwata, Akihiro Oyama, Masato Abei, Tatsuo Gondo, Takehiko Ogawa, Yuichi Tamaki, Hiromi Nishiyama, Hidenori Akutsu, Masamitsu Tanaka, Yoshiaki Ide, Kunihiro Kurihara, Akio Asai, Taka Nakahara, Yukio Nakamura, Toshihide Ito, Kazuo Umezawa, Kazutoshi Shibuya, Noriko Kunifuji, Masanori Nasu, Kumiko Tsuboi, Masanori Hasegawa, and Nobuyuki Tanaka

Subjects
Cancer Research, medicine.medical_specialty, Family medicine, medicine, Reproductive medicine, Cell Biology, Human cell, Biology, Stem cell
Published
2010
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3. Establishment and characterization of a novel cell line derived from a human small cell lung carcinoma that secretes parathyroid hormone, parathyroid hormone-related protein, and pro-opiomelanocortin

Author

Kumiko Tsuboi, Masako Shimojo, Toshiaki Tachibana, Kazutoshi Shibuya, Hajime Ueshiba, Hisashi Hashimoto, Mayumi Ishikawa, Gen Yoshino, and Kazuhiro Kimura

Subjects
endocrine system, Cancer Research, medicine.medical_specialty, Parathyroid hormone-related protein, Cell, Parathyroid hormone, Cell Biology, Biology, Corticotropin-releasing hormone, Endocrinology, medicine.anatomical_structure, Cell culture, Internal medicine, medicine, Calcium-sensing receptor, Receptor, hormones, hormone substitutes, and hormone antagonists, Fetal bovine serum
Abstract

There are few case reports describing small cell lung carcinoma (SCLC), which secrete parathyroid hormone (PTH)-related protein (PTHrP) and result in hypercalcemia. We have established a novel cell line, derived from a 37-year-old woman with SCLC, which produced PTH, PTH-rP, and a part of proopiomelanocortin (POMC), and led to hypercalcemia. The cell line, named SS-1, was grown as floating cell clusters in DMEM/F12 medium supplemented with 10% fetal bovine serum and had a population doubling time of 72 h. The modal chromosome number was 47 (88%); marker chromosomes were not observed. The SS-1 cell line secreted not only PTHrP but also PTH, and both were decreased by CaCl2 administration. Decreasing the concentration of Ca++ in the growth medium stimulated the secretion of both PTHrP and PTH. The cell line had calcium sensing receptor (Cas-R). Since PTHrP and PTH secretion from the SS-1 cells was related to Ca++ concentration in the growth medium, the cell line might be useful for the study of PTH-rP and PTH regulation as well as for SCLC analysis. In addition, the cells secreted N terminal POMC, the precursor of adrenocorticotropic hormone, in response to stimulation with corticotropin releasing hormone. In summary, we established a novel cell line, SS-1 from SCLC, which produced PTHrP, PTH and N terminal POMC.

Published
2010
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4. Abstract of Poster Presentation

Author

Hiroshi Uchida, Tetsuo Maruyama, Jkuko Sugiura, Takashi Kajitani, Touru Arase, Masanori Ono, Takashi Nagashima, Hirotaka Masuda, Hironori Asada, Yasunori Yoshimura, Soichiro Saito, Mila Ghosh, Keiko Morita, Masanao Miwa, Takashi Hirano, Takeshi Todoroki, Kento Kanao, Mototsugu Ohya, Masaru Murai, Junko Ohnuki, Lee Wee Khor, Naoya Kamiyama, Nobumoto Tomioka, Takahito Nakagawa, Masato Takahashi, Satoru Todo, Moriaki Kusakabe, Satoshi Aotsuka, Jun Inoue, Kyouichi Matsuba, Hisashi Hashimoto, Seiji Isonishi, Makoto Yasuda, Hiroshi Ishikawa, Masatsugu Ueda, Yoshito Terai, Hiroyuki Yamaguchi, Minoru Ueki, Shunro Uchinokura, Shiro Miyata, Tsuyoshi Fukushima, Hiroshi Itoh, Shinichi Nakano, Shinichiro Wakisaka, Hiroaki Kataoka, Satoshi Ohi, Isao Tabei, Tetsuya Hirabayashi, Kozou Ninomiya, Ayumi Ichikawa, Tomomi Ogata, Toshiyuki Tachibana, Kahei Sato, Katsuhiko Yanaga, Tomoharu Tamagawa, Megumi Iguchi, Yuko Tokieda, Isamu Ishiwata, Isao Ono, Kazushige Kiguchi, Chieko Ishiwata, Emiko Ishiwata, Masayuki Soma, Hiroko Nakamura, Jinji Mizuno, Youichi Fueta, Hirokazu Kamakura, Yoshinobu Murayama, Sadao Omata, Kazuyuki Akaishi, Hiroaki Inui, Jinii Mizuno, Serge Ostrovidov, Yasuyuki Sakai, Teruo Fujii, Natsumi Watanabe, Kazuhiro Hirayma, Eiko Kuriki, Mamoru Kobayashi, Takushi Yakuwa, Naoki Okamoto, Yorino Sato, Fumi Tanaka, Ai Kazami, Hisataka Hasegawa, Naoki Tanaka, Yasuyuki Araki, Midori Yoshizawa, Yasuhisa Araki, Eriko Sakaguchi, Kouichi Tomita, Kozo Ninomiya, Yuichi Ishida, Toshiaki Tachibana, Kumiko Tsuboi, Mayumi Ishikawa, Hajime Ueshiba, Shinzo Kitahara, Gen Yoshino, Samu Ishiwata, Kenichi Miharada, Kazuhiro Sudo, Yukio Nakamura, Qiang Li, Takashi Ryu, Keiichi Azuma, Naoki Hosaka, Susumu Ikehara, Keiji Kawamoto, Toshio Hamatani, Hideyuki Okano, Yumi Matsuzaki, Matsuo Yamamoto, Kenji Sakoda, Yoichi Negishi, Hideki Sekiya, Yusuke Sakiyama, Kazunari Suzuki, Tomoya Yano, Yukiko Fukawa, Koichi Node, Yuichi Izumi, Makoto Kobayashi, Takamasa Takagi, Noriko Takahashi, Masashi Mitsui, Reiichiro Murayama, Shunichiro Moritaka, Ayuko Tsurumi, Iyou Hayashi, Yukie Hayashi, Yoshimasa Okamatsu, Ken-ichi Miharada, Takashi Hiroyama, Tsuyoshi Fujioka, Toshiro Nagasawa, Nao Suzuki, Kimiko Orikawa, Yutaka Tamada, Atsushi Suzuki, Nobuyuki Susumu, Katsumi Tsukazaki, Makio Mukai, Kyoko Kojima-Aikawa, Isao Ishida, Daisuke Aoki, Yoichi Kobayashi, Noriyuki Takahashi, Tatsuru Ohara, Yoshiko Okuda, Sojiro Sato, Bunpei Ishizuka, Akinori Sato, Keiichi Ito, Mototsugu Ooya, Takako Asano, Makoto Sumitomo, Yutaka Horiguchi, Tomohiko Asano, Masamichi Hayakawa, Naoki Sasaki, Tsunekazu Kita, Sanshiro Okamoto, Masashi Takano, Kazuya Kudoh, Kenichi Furuya, Yoshihiro Kikuchi, Koichi Nariai, Tetsuya Yoshikawa, Makoto Mitsunaga, Makoto Sumi, Yoko Yumoto, Yasuo Mabashi, Yoshihisa Namiki, Akihito Tsubota, Kiyotaka Fujise, Hiroshi Takahashi, Hideki Harada, Shinya Suzu, Takaaki Ito, Seiji Okada, Nagazumi Suzuki, Kazu Ueda, Kyosuke Yamada, Tadao Tanaka, Kan Kondo, Yutaka Shimada, Yoshihiro Nakagami, Teiichiro Aoyagi, Tatsuo Gondo, Noboru Sakamoto, Yoshio Ohno, Shouji Koga, Kazunori Namiki, Kunihiko Yoshioka, Makoto Ohori, Tadashi Hatano, Masaaki Tachibana, Mari Watanabe, Yasuna Wada, and Hiroshi Mizuhara

Subjects
Cancer Research, medicine.medical_specialty, business.industry, General surgery, medicine, Reproductive medicine, Cell Biology, Presentation (obstetrics), Stem cell, business
Published
2005
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5. Establishment and characterization of a novel cell line derived from a human small cell lung carcinoma that secretes parathyroid hormone, parathyroid hormone-related protein, and pro-opiomelanocortin

Author

Mayumi, Ishikawa, Kazuhiro, Kimura, Toshiaki, Tachibana, Hisashi, Hashimoto, Masako, Shimojo, Hajime, Ueshiba, Kumiko, Tsuboi, Kazutoshi, Shibuya, and Gen, Yoshino

Subjects
Adult, Lung Neoplasms, Pro-Opiomelanocortin, Parathyroid Hormone, Cell Line, Tumor, Karyotyping, Hypercalcemia, Parathyroid Hormone-Related Protein, Humans, Female, Microscopy, Phase-Contrast, Receptors, Calcium-Sensing, Small Cell Lung Carcinoma
Abstract

There are few case reports describing small cell lung carcinoma (SCLC), which secrete parathyroid hormone (PTH)-related protein (PTHrP) and result in hypercalcemia. We have established a novel cell line, derived from a 37-year-old woman with SCLC, which produced PTH, PTH-rP, and a part of proopiomelanocortin (POMC), and led to hypercalcemia. The cell line, named SS-1, was grown as floating cell clusters in DMEM/F12 medium supplemented with 10% fetal bovine serum and had a population doubling time of 72 h. The modal chromosome number was 47 (88%); marker chromosomes were not observed. The SS-1 cell line secreted not only PTHrP but also PTH, and both were decreased by CaCl(2) administration. Decreasing the concentration of Ca(++) in the growth medium stimulated the secretion of both PTHrP and PTH. The cell line had calcium sensing receptor (Cas-R). Since PTHrP and PTH secretion from the SS-1 cells was related to Ca(++) concentration in the growth medium, the cell line might be useful for the study of PTH-rP and PTH regulation as well as for SCLC analysis. In addition, the cells secreted N terminal POMC, the precursor of adrenocorticotropic hormone, in response to stimulation with corticotropin releasing hormone. In summary, we established a novel cell line, SS-1 from SCLC, which produced PTHrP, PTH and N terminal POMC.

Published
2010

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