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Start Over Author "Shiro Miyata" Journal human cell
3 results on '"Shiro Miyata"'

1. Abstract of Poster Presentation

Author

Hiroshi Uchida, Tetsuo Maruyama, Jkuko Sugiura, Takashi Kajitani, Touru Arase, Masanori Ono, Takashi Nagashima, Hirotaka Masuda, Hironori Asada, Yasunori Yoshimura, Soichiro Saito, Mila Ghosh, Keiko Morita, Masanao Miwa, Takashi Hirano, Takeshi Todoroki, Kento Kanao, Mototsugu Ohya, Masaru Murai, Junko Ohnuki, Lee Wee Khor, Naoya Kamiyama, Nobumoto Tomioka, Takahito Nakagawa, Masato Takahashi, Satoru Todo, Moriaki Kusakabe, Satoshi Aotsuka, Jun Inoue, Kyouichi Matsuba, Hisashi Hashimoto, Seiji Isonishi, Makoto Yasuda, Hiroshi Ishikawa, Masatsugu Ueda, Yoshito Terai, Hiroyuki Yamaguchi, Minoru Ueki, Shunro Uchinokura, Shiro Miyata, Tsuyoshi Fukushima, Hiroshi Itoh, Shinichi Nakano, Shinichiro Wakisaka, Hiroaki Kataoka, Satoshi Ohi, Isao Tabei, Tetsuya Hirabayashi, Kozou Ninomiya, Ayumi Ichikawa, Tomomi Ogata, Toshiyuki Tachibana, Kahei Sato, Katsuhiko Yanaga, Tomoharu Tamagawa, Megumi Iguchi, Yuko Tokieda, Isamu Ishiwata, Isao Ono, Kazushige Kiguchi, Chieko Ishiwata, Emiko Ishiwata, Masayuki Soma, Hiroko Nakamura, Jinji Mizuno, Youichi Fueta, Hirokazu Kamakura, Yoshinobu Murayama, Sadao Omata, Kazuyuki Akaishi, Hiroaki Inui, Jinii Mizuno, Serge Ostrovidov, Yasuyuki Sakai, Teruo Fujii, Natsumi Watanabe, Kazuhiro Hirayma, Eiko Kuriki, Mamoru Kobayashi, Takushi Yakuwa, Naoki Okamoto, Yorino Sato, Fumi Tanaka, Ai Kazami, Hisataka Hasegawa, Naoki Tanaka, Yasuyuki Araki, Midori Yoshizawa, Yasuhisa Araki, Eriko Sakaguchi, Kouichi Tomita, Kozo Ninomiya, Yuichi Ishida, Toshiaki Tachibana, Kumiko Tsuboi, Mayumi Ishikawa, Hajime Ueshiba, Shinzo Kitahara, Gen Yoshino, Samu Ishiwata, Kenichi Miharada, Kazuhiro Sudo, Yukio Nakamura, Qiang Li, Takashi Ryu, Keiichi Azuma, Naoki Hosaka, Susumu Ikehara, Keiji Kawamoto, Toshio Hamatani, Hideyuki Okano, Yumi Matsuzaki, Matsuo Yamamoto, Kenji Sakoda, Yoichi Negishi, Hideki Sekiya, Yusuke Sakiyama, Kazunari Suzuki, Tomoya Yano, Yukiko Fukawa, Koichi Node, Yuichi Izumi, Makoto Kobayashi, Takamasa Takagi, Noriko Takahashi, Masashi Mitsui, Reiichiro Murayama, Shunichiro Moritaka, Ayuko Tsurumi, Iyou Hayashi, Yukie Hayashi, Yoshimasa Okamatsu, Ken-ichi Miharada, Takashi Hiroyama, Tsuyoshi Fujioka, Toshiro Nagasawa, Nao Suzuki, Kimiko Orikawa, Yutaka Tamada, Atsushi Suzuki, Nobuyuki Susumu, Katsumi Tsukazaki, Makio Mukai, Kyoko Kojima-Aikawa, Isao Ishida, Daisuke Aoki, Yoichi Kobayashi, Noriyuki Takahashi, Tatsuru Ohara, Yoshiko Okuda, Sojiro Sato, Bunpei Ishizuka, Akinori Sato, Keiichi Ito, Mototsugu Ooya, Takako Asano, Makoto Sumitomo, Yutaka Horiguchi, Tomohiko Asano, Masamichi Hayakawa, Naoki Sasaki, Tsunekazu Kita, Sanshiro Okamoto, Masashi Takano, Kazuya Kudoh, Kenichi Furuya, Yoshihiro Kikuchi, Koichi Nariai, Tetsuya Yoshikawa, Makoto Mitsunaga, Makoto Sumi, Yoko Yumoto, Yasuo Mabashi, Yoshihisa Namiki, Akihito Tsubota, Kiyotaka Fujise, Hiroshi Takahashi, Hideki Harada, Shinya Suzu, Takaaki Ito, Seiji Okada, Nagazumi Suzuki, Kazu Ueda, Kyosuke Yamada, Tadao Tanaka, Kan Kondo, Yutaka Shimada, Yoshihiro Nakagami, Teiichiro Aoyagi, Tatsuo Gondo, Noboru Sakamoto, Yoshio Ohno, Shouji Koga, Kazunori Namiki, Kunihiko Yoshioka, Makoto Ohori, Tadashi Hatano, Masaaki Tachibana, Mari Watanabe, Yasuna Wada, and Hiroshi Mizuhara

Subjects
Cancer Research, medicine.medical_specialty, business.industry, General surgery, medicine, Reproductive medicine, Cell Biology, Presentation (obstetrics), Stem cell, business
Published
2005
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2. Roles of Kunitz domains in the anti-invasive effect of hepatocyte growth factor activator inhibitor type 1 in human glioblastoma cells

Author

Hideo Takeshima, Hiroyuki Tanaka, Tsuyoshi Fukushima, Shiro Miyata, Kazuyo Kohama, and Hiroaki Kataoka

Subjects
Cancer Research, animal structures, Serine Proteinase Inhibitors, Hepsin, Amino Acid Motifs, Proteinase Inhibitory Proteins, Secretory, virus diseases, Cell Biology, Hepatocyte Growth Factor Activator, Biology, Molecular biology, In vitro, Protease inhibitor (biology), Protein Structure, Tertiary, Type IV collagen, Extracellular, biology.protein, medicine, Tumor Cells, Cultured, Humans, Matriptase, Neoplasm Invasiveness, Kunitz domain, Glioblastoma, medicine.drug
Abstract

Hepatocyte growth factor activator inhibitor type 1 (HAI-1) is a membrane-bound serine proteinase inhibitor having two extracellular Kunitz-type proteinase inhibitor domains (KD) namely KD-1 and KD-2. It efficiently inhibits hepatocyte growth factor activator, matriptase, hepsin, prostasin and trypsin. We have previously reported that the expression of HAI-1 suppresses the in vitro invasive capability of human glioblastoma cells. In this study we examined the role of each KD in the anti-invasive effect of HAI-1. Engineered over-expression of the mature membrane-form HAI-1 suppressed in vitro fibrin gel invasion of two human glioblastoma cell lines, U251 and YKG-1. The migratory activity on type IV collagen was also suppressed by the HAI-1 expression. These effects were not affected by the deletion of intracytoplasmic domain of HAI-1. A truncated secreted form of HAI-1 also suppressed in vitro invasion of the cells, indicating that the extracellular portion of HAI-1 was responsible for the anti-invasive effect. To determine the roles of each KD in the anti-invasive effect of HAI-1 in vitro, we constructed expression plasmids for HAI-1 with or without mutation at the P1 position of the reactive site of each KD. The results revealed that the proteinase inhibitor activity of N-terminal KD (KD-1) is responsible for the anti-invasion effect of HAI-1.

Published
2007

3. Establishment and characterization of a new human glioblastoma cell line, NYGM

Author

Hiroaki Kataoka, Shinichiro Wakisaka, Yoshitsugu Nuki, Hiroshi Itoh, Shinichi Nakano, Shunro Uchinokura, Shiro Miyata, Tsuyoshi Fukushima, Yutaka Akiyama, and Ryouichi Hamasuna

Subjects
Cancer Research, C-Met, Cell Culture Techniques, Biology, Ligands, chemistry.chemical_compound, Mice, Cell Line, Tumor, Proto-Oncogene Proteins, medicine, Doubling time, Animals, Humans, Aged, Mice, Inbred BALB C, c-Mer Tyrosine Kinase, Brain Neoplasms, Hepatocyte Growth Factor, HGF Activator, Serine Endopeptidases, Chromosome Mapping, Receptor Protein-Tyrosine Kinases, Cell Biology, Ligand (biochemistry), medicine.disease, Molecular biology, chemistry, Cell culture, Hepatocyte growth factor, Female, Stem cell, Glioblastoma, Cell Division, Neoplasm Transplantation, medicine.drug
Abstract

A cell line designated NYGM was established from a human cerebral glioblastoma multiforme (GBM) obtained from a 75-year-old Japanese woman. The cell line has grown slowly without interruption and has been propagated continuously by serial passages (more than 80 passage) during the past 3 years. The cultured cells were fusiform or polyhedral in shape. The population doubling time was 24 hours. The chromosomal number varied between 77 and 88, with modal chromosomal number of 84. NYGM cells concomitantly expressed MET receptor tyrosine kinase (a product of c-met protooncogene) and its ligand HGF/SF (hepatocyte growth factor/scatter factor), as well as HGF activator and HGF activator inhibitors. The cells might be useful for the study of pericellular regulation of HGF/SF-MET signaling and HGF activation of GBM cells.

Published
2005

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