Search

Aim Administration of anti-MHC class I (H-2Kb) leads to induction of transcription factor Zinc finger and BTB domain containing 7A (Zbtb7a) leading to inflammatory cascade and chronic rejection in a murine obliterative airway disease model. Development of DSA and Abs to lung-associated self-antigens (K-alpla-1 Tubulin (K α 1T) and Collagen V (Col-V) are significant risk factors in development of bronchiolitis obliterans syndrome (BOS) following human lung transplantation (LTx). The current study focused on the role of Zbtb7a in development of BOS following human LTx. Methods We analyzed 25 LTx patients that developed BOS years after LTx as well as 10 stable LTx performed at Barnes-Jewish Washington University. Kinetics of Zbtb7a expression was studied in bronchoalveolar lavage (BAL) cells. Quantitative PCR analysis was performed to evaluate mRNA levels and data was compared with the DSA, autoantibody and immunosuppression. Results Increases in the Zbtb7a expression was observed in BAL cells collected at visits immediately prior to clinical BOS diagnosis compared with earlier visits. Zbtb7a expression was significantly ( p < 0.01) increased (150%) in pre-BOS BAL cells. On the contrary, stable patients did not demonstrate any change in Zbtb7a levels. All of the BOS+ patients had developed DSA and Abs to K α 1T and Col-V. Zbtb7a expression in 13 patients who received Ab directed therapy following DSA demonstrated significant reduction along with decrease in DSA and Abs to lung self-antigens. Therefore, Zbtb7a activation is an important inducer of the DSA and autoantibody. Conclusions Up regulation of Zbtb7a was positively associated with de novo DSA and Abs to lung-associated self-antigens. Ab directed therapy significantly reduced Zbtb7a expression. We propose that Zbtb7a may be used as a prognostic marker in evaluating Ab-induced inflammation in the lung as well as efficacy of Ab directed therapy following development of DSA. [ABSTRACT FROM AUTHOR]

Aim: De novo autoantibodies (autoAbs) against lung restricted autoantigens, namely k-alpha 1 tubulin (KAT) and collagen type V (colV), develop following transplant but their role in allograft rejection remains unclear. Using the murine model of lung transplant we investigated whether these autoAbs can abrogate immunosuppression mediated tolerance of lung allografts and induce rejection of syngeneic grafts. Methods: Abs to KAT, ColV and isotype control (n =5/group) were administered in Bl/6 murine recipients of MHC-mismatched Balb/c single left lung allografts that were tolerized using costimulatory blockade (MR1 and CTLA4-Ig). ELISPOT was used to analyze antigen specific T cells. Graft rejection was characterized using histochemistry. Syngeneic single left lung transplants were performed in B/6 mice following which Abs to KAT, ColV and isotype control were injected. Results: Lung allografts showed no rejection following co-stimulatory blockade. Administration of either KAT or colV autoAbs, but not isotype control, led to allograft rejection (but not native lungs) by day 45. This correlated with infiltration of IFN and IL-17 secreting Tcells specific to the lung-restricted autoantigens (but not Collagen II) as well as donor MHC antigens (but not against third party MHC antigens). Either KAT or colV autoAbs alone induced immunity against the other lung restricted autoantigen as well but not against non-lung antigen Collagen II. Administration of either colV or KAT autoAbs led to the rejection of syngeneic left lung grafts (but not native lungs) that was associated with infiltration of autoantigen specific IFN and IL-17 secreting T cells. Conclusions: De novo autoAbs can abrogate immunosuppression mediated lung allograft tolerance. Autoabs can lead to graft rejection in the absence of alloimmunity as evident by rejection of syngeneic grafts. AutoAbs also lead to epitope spreading and development of additional autoimmune responses against target tissue-restricted as well as donor MHC antigens. [ABSTRACT FROM AUTHOR]