1. Siglec-10 expression is up-regulated in activated human CD4 + T cells.
- Author
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Bandala-Sanchez E, Bediaga NG, Naselli G, Neale AM, and Harrison LC
- Subjects
- CD4-Positive T-Lymphocytes immunology, CD52 Antigen immunology, CD52 Antigen metabolism, Humans, Lectins immunology, Lymphocyte Activation immunology, Receptors, Cell Surface immunology, Up-Regulation, CD4-Positive T-Lymphocytes metabolism, Lectins metabolism, Receptors, Cell Surface metabolism
- Abstract
Most sialic acid-binding immunoglobulin-like lectins (Siglecs) suppress immune cell function but are expressed at low levels on human T cells. We found that soluble CD52 inhibited T cell signalling by ligating Siglec-10, but the presence of Siglec-10 on human T cells has been questioned. To address this concern, we examined the expression of Siglec-10 at the RNA and protein level in human CD4
+ T cells. Analysis by RNAseq, qPCR and flow cytometry demonstrated that, in contrast to other Siglecs, after activation of CD4+ T cells Siglec-10 was selectively upregulated in a subset of cells also high for CD52 expression. This observation is consistent with a homeostatic role for Siglec-10 in human CD4+ T cells., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.)- Published
- 2020
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