1. HLA-G is expressed in intestinal samples of ulcerative colitis and Crohn's disease patients and HLA-G5 expression is differentially correlated with TNF and IL-10 cytokine expression.
- Author
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Gomes RG, Brito CAA, Martinelli VF, Santos RND, Gomes FODS, Peixoto CA, Crispim JO, Diniz GTN, Donadi EA, and Lucena-Silva N
- Subjects
- Adolescent, Adult, Aged, Disease Progression, Female, Humans, Immunohistochemistry, Immunomodulation, Interleukin-10 genetics, Interleukin-10 metabolism, Intestines pathology, Male, Middle Aged, Polymerase Chain Reaction, RNA, Messenger analysis, Tumor Necrosis Factor-alpha genetics, Tumor Necrosis Factor-alpha metabolism, Young Adult, Colitis, Ulcerative immunology, Crohn Disease immunology, Epithelial Cells physiology, HLA-G Antigens metabolism, Intestinal Mucosa metabolism
- Abstract
HLA-G is an immunomodulatory molecule that can be produced by epithelial cells. Considering that TNF and IL-10 participate in bowel inflammatory disorders and that both cytokines modulate HLA-G, we evaluated HLA-G, TNF and IL-10 mRNA expression by qPCR and HLA-G protein levels by immunohistochemistry in two intestinal samples exhibiting different degree of inflammation within a patient suffering from Crohn's disease (CD) or ulcerative colitis (UC). Tissue HLA-G5 (P < 0.0001), TNF (P = 0.0004) and IL-10 (P = 0.0169) mRNA expression levels were higher in intestinal areas exhibiting intense inflammation compared to areas of low inflammation, and HLA-G protein levels were not associated with degree of mucosal inflammation. In CD, the expression of TNF was correlated with IL-10 in low inflamed areas, exhibiting a TNF:IL-10 ratio = 3, but in inflamed areas the ratio increased to 9-folds. In UC, the expression of TNF was correlated to IL-10, irrespective of the inflammation grade, with little variation of the TNF:IL-10 ratio in the various inflamed areas. TNF and IL-10 expression was correlated with HLA-G5 expression in mild inflamed areas. Both CD and UC samples exhibited gene and protein expression of HLA-G; and the HLA-G5 expression is differentially correlated with TNF and IL-10 levels depending on the type of the underlying inflammatory bowel disorder., (Copyright © 2018 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.)
- Published
- 2018
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