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Start Over Author "De Placido, G" Journal human reproduction
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5. REPRODUCTIVE ENDOCRINOLOGY

Author

Karasu, Y., primary, Dilbaz, B., additional, Demir, B., additional, Dilbaz, S., additional, Secilmis Kerimoglu, O., additional, Ercan, C. M., additional, Keskin, U., additional, Korkmaz, C., additional, Duru, N. K., additional, Ergun, A., additional, de Zuniga, I., additional, Horton, M., additional, Oubina, A., additional, Scotti, L., additional, Abramovich, D., additional, Pascuali, N., additional, Tesone, M., additional, Parborell, F., additional, Bouzas, N., additional, Yang, X. H., additional, Chen, S. L., additional, Chen, X., additional, Ye, D. S., additional, Zheng, H. Y., additional, Nyboe Andersen, A., additional, Lauritsen, M. P., additional, Thuesen, L. L., additional, Khodadadi, M., additional, Shivabasavaiah, S., additional, Mozafari, R., additional, Ansari, Z., additional, Hamdine, O., additional, Broekmans, F., additional, Eijkemans, M. J. C., additional, Cohlen, B. J., additional, Verhoeff, A., additional, van Dop, P. A., additional, Bernardus, R. E., additional, Lambalk, C. B., additional, Oosterhuis, G. J. E., additional, Holleboom, C., additional, van den Dool-Maasland, G. C., additional, Verburg, H. J., additional, van der Heijden, P. F. M., additional, Blankhart, A., additional, Fauser, B. C. J. M., additional, Laven, J. S. E., additional, Macklon, N. S., additional, Agudo, D., additional, Lopez, C., additional, Alonso, M., additional, Huguet, E., additional, Bronet, F., additional, Garcia-Velasco, J. A., additional, Requena, A., additional, Gonzalez Comadran, M., additional, Checa, M. A., additional, Duran, M., additional, Fabregues, F., additional, Carreras, R., additional, Ersahin, A., additional, Kahraman, S., additional, Kavrut, M., additional, Gorgen, B., additional, Acet, M., additional, Dokuzeylul, N., additional, Aybar, F., additional, Lim, S. Y., additional, Park, J. C., additional, Bae, J. G., additional, Kim, J. I., additional, Rhee, J. H., additional, Mahran, A., additional, Abdelmeged, A., additional, El-Adawy, A., additional, Eissa, M., additional, Darne, J., additional, Shaw, R. W., additional, Amer, S. A., additional, Dai, A., additional, Yan, G., additional, He, Q., additional, Hu, Y., additional, Sun, H., additional, Ferrero, H., additional, Gomez, R., additional, Garcia-Pascual, C. M., additional, Simon, C., additional, Gaytan, F., additional, Pellicer, A., additional, Garcia Pascual, C. M., additional, Zimmermann, R. C., additional, Madani, T., additional, Mohammadi Yeganeh, L., additional, Khodabakhshi, S. H., additional, Akhoond, M. R., additional, Hasani, F., additional, Monzo, C., additional, Haouzi, D., additional, Assou, S., additional, Dechaud, H., additional, Hamamah, S., additional, Amer, S., additional, Mahran, M., additional, Shaw, R., additional, Lan, V., additional, Nhu, G., additional, Tuong, H., additional, Mahmoud Youssef, M. A., additional, Aboulfoutouh, I., additional, Al-inany, H., additional, Van Der Veen, F., additional, Van Wely, M., additional, Zhang, Q., additional, Fang, T., additional, Wu, S., additional, Zhang, L., additional, Wang, B., additional, Li, X., additional, Ding, L., additional, Day, A., additional, Fulford, B., additional, Boivin, J., additional, Alanbay, I., additional, Sakinci, M., additional, Coksuer, H., additional, Ozturk, M., additional, Tapan, S., additional, Chung, C. K., additional, Chung, Y., additional, Seo, S., additional, Aksoy, S., additional, Yakin, K., additional, Caliskan, S., additional, Salar, Z., additional, Ata, B., additional, Urman, B., additional, Devroey, P., additional, Arce, J. C., additional, Harrison, K., additional, Irving, J., additional, Osborn, J., additional, Harrison, M., additional, Fusi, F., additional, Arnoldi, M., additional, Cappato, M., additional, Galbignani, E., additional, Galimberti, A., additional, Zanga, L., additional, Frigerio, L., additional, Taghavi, S. A., additional, Ashrafi, M., additional, Karimian, L., additional, Mehdizadeh, M., additional, Joghataie, M., additional, Aflatoonian, R., additional, Xu, B., additional, Cui, Y. G., additional, Gao, L. L., additional, Diao, F. Y., additional, Li, M., additional, Liu, X. Q., additional, Liu, J. Y., additional, Jiang, F., additional, Jee, B. C., additional, Yi, G., additional, Kim, J. Y., additional, Suh, C. S., additional, Kim, S. H., additional, Liu, S., additional, Cai, L. B., additional, Liu, J. J., additional, Ma, X., additional, Geenen, E., additional, Bots, R. S. G. M., additional, Smeenk, J. M. J., additional, Chang, E., additional, Lee, W., additional, Seok, H., additional, Kim, Y., additional, Han, J., additional, Yoon, T., additional, Lazaros, L., additional, Xita, N., additional, Zikopoulos, K., additional, Makrydimas, G., additional, Kaponis, A., additional, Sofikitis, N., additional, Stefos, T., additional, Hatzi, E., additional, Georgiou, I., additional, Atilgan, R., additional, Kumbak, B., additional, Sahin, L., additional, Ozkan, Z. S., additional, Simsek, M., additional, Sapmaz, E., additional, Karacan, M., additional, Alwaeely, F. A., additional, Cebi, Z., additional, Berberoglugil, M., additional, Ulug, M., additional, Camlibel, T., additional, Yelke, H., additional, Kamalak, Z., additional, Carlioglu, A., additional, Akdeniz, D., additional, Uysal, S., additional, Inegol Gumus, I., additional, Ozturk Turhan, N., additional, Regan, S., additional, Yovich, J., additional, Stanger, J., additional, Almahbobi, G., additional, Kara, M., additional, Aydin, T., additional, Turktekin, N., additional, Youssef, M., additional, Al-Inany, H., additional, van der Veen, F., additional, van Wely, M., additional, Hart, R., additional, Doherty, D., additional, Frederiksen, H., additional, Keelan, J., additional, Pennell, C., additional, Newnham, J., additional, Skakkebaek, N., additional, Main, K., additional, Salem, H. T., additional, Ismail, A. a., additional, Viola, M., additional, Siebert, T. I., additional, Steyn, D. W., additional, Kruger, T. F., additional, Robin, G., additional, Dewailly, D., additional, Thomas, P., additional, Leroy, M., additional, Lefebvre, C., additional, soudan, B., additional, Pigny, P., additional, Decanter, C., additional, ElPrince, M., additional, Wang, F., additional, Zhu, Y., additional, Huang, H., additional, Valdez Morales, F., additional, Vital Reyes, V., additional, Mendoza Rodriguez, A., additional, Gamboa Dominguez, A., additional, Cerbon, M., additional, Aizpurua, J., additional, Ramos, B., additional, Luehr, B., additional, Moragues, I., additional, Rogel, S., additional, Cil, A. P., additional, Guler, Z. B., additional, Kisa, U., additional, Albu, A., additional, Radian, S., additional, Grigorescu, F., additional, Albu, D., additional, Fica, S., additional, Al Boghdady, L., additional, Ghanem, M. E., additional, Hassan, M., additional, Helal, A. S., additional, Ozdogan, S., additional, Ozdegirmenci, O., additional, Cinar, O., additional, Goktolga, U., additional, Seeber, B., additional, Tsybulyak, I., additional, Bottcher, B., additional, Grubinger, T., additional, Czech, T., additional, Wildt, L., additional, Wojcik, J., additional, Howles, C. M., additional, Destenaves, B., additional, Arriagada, P., additional, Tavmergen, E., additional, Sahin, G., additional, Akdogan, A., additional, Levi, R., additional, Goker, E. N. T., additional, Loft, A., additional, Smitz, J., additional, Ricciardi, L., additional, Di Florio, C., additional, Busacca, M., additional, Gagliano, D., additional, Immediata, V., additional, Selvaggi, L., additional, Romualdi, D., additional, Guido, M., additional, Bouhanna, P., additional, Salama, S., additional, Kamoud, Z., additional, Torre, A., additional, Paillusson, B., additional, Fuchs, F., additional, Bailly, M., additional, Wainer, R., additional, Tagliaferri, V., additional, Tartaglia, C., additional, Cirella, E., additional, Aflatoonian, A., additional, Eftekhar, M., additional, Mohammadian, F., additional, Yousefnejad, F., additional, De Cicco, S., additional, Campagna, G., additional, Depalo, R., additional, Lippolis, C., additional, Vacca, M., additional, Nardelli, C., additional, Cavallini, A., additional, Panic, T., additional, Mitulovic, G., additional, Franz, M., additional, Sator, K., additional, Tschugguel, W., additional, Pietrowski, D., additional, Hildebrandt, T., additional, Cupisti, S., additional, Giltay, E. J., additional, Gooren, L. J., additional, Oppelt, P. G., additional, Hackl, J., additional, Reissmann, C., additional, Schulze, C., additional, Heusinger, K., additional, Attig, M., additional, Hoffmann, I., additional, Beckmann, M. W., additional, Dittrich, R., additional, Mueller, A., additional, Sharma, S., additional, Singh, S., additional, Chakravarty, A., additional, Sarkar, A., additional, Rajani, S., additional, Chakravarty, B. N., additional, Ozturk, E., additional, Isikoglu, S., additional, Kul, S., additional, Hillensjo, T., additional, Witjes, H., additional, Elbers, J., additional, Mannaerts, B., additional, Gordon, K., additional, Krasnopolskaya, K., additional, Galaktionova, A., additional, Gorskaya, O., additional, Kabanova, D., additional, Venturella, R., additional, Morelli, M., additional, Mocciaro, R., additional, Capasso, S., additional, Cappiello, F., additional, Zullo, F., additional, Monterde, M., additional, Marzal, A., additional, Vega, O., additional, Rubio-Rubio, J. M., additional, Diaz-Garcia, C., additional, Kolibianakis, E., additional, Griesinger, G., additional, Yding Andersen, C., additional, Ocal, P., additional, Guralp, O., additional, Aydogan, B., additional, Irez, T., additional, Cetin, M., additional, Senol, H., additional, Erol, N., additional, Rombauts, L., additional, Van Kuijk, J., additional, Montagut, J., additional, Nogueira, D., additional, Porcu, G., additional, Chomier, M., additional, Giorgetti, C., additional, Nicollet, B., additional, Degoy, J., additional, Lehert, P., additional, Alviggi, C., additional, De Rosa, P., additional, Vallone, R., additional, Picarelli, S., additional, Coppola, M., additional, Conforti, A., additional, Strina, I., additional, Di Carlo, C., additional, De Placido, G., additional, Haeberle, L., additional, Demirtas, O., additional, Fatemi, H., additional, Shapiro, B. S., additional, Mannaerts, B. M., additional, Chimote, M. N., additional, Mehta, B. N., additional, Chimote, N. N., additional, Nath, N. M., additional, Chimote, N. M., additional, Karia, S., additional, Bonifacio, M., additional, Bowman, M., additional, McArthur, S., additional, Jung, J., additional, Cho, S., additional, Choi, Y., additional, Lee, B., additional, Lee, K. H., additional, Kim, C. H., additional, Kwon, S. K., additional, Kang, B. M., additional, Jung, K. S., additional, Basios, G., additional, Trakakis, E., additional, Hatziagelaki, E., additional, Vaggopoulos, V., additional, Tsiavou, A., additional, Panagopoulos, P., additional, Chrelias, C., additional, Kassanos, D., additional, Sarhan, A., additional, Elsamanoudy, A., additional, Harira, M., additional, Dogan, S., additional, Bozdag, G., additional, Esinler, I., additional, Polat, M., additional, and Yarali, H., additional

Published
2012
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11. Rescue of IVF cycles by HMG in pituitary down-regulated normogonadotrophic young women characterized by a poor initial response to recombinant FSH.

Author

De Placido, Giuseppe, Mollo, Antonio, Alviggi, Carlo, Strina, Ida, Varricchio, Maria Teresa, Ranieri, Antonio, Colacurci, Nicola, Tolino, Achille, Wilding, Martin, De Placido, G, Mollo, A, Alviggi, C, Strina, I, Varricchio, M T, Ranieri, A, Colacurci, N, Tolino, A, and Wilding, M

Abstract

Background: The aim of this study was to investigate the effects of adding human menopausal gonadotrophin (HMG) during controlled ovarian stimulation in normoovulatory normogonadotrophic patients showing an initial suboptimal response to a standardized long protocol therapy with recombinant FSH (rFSH) (300 IU/day).Methods: A total of 43 such patients were randomized in two groups. In Group A, 150 IU rFSH was substituted by 150 IU HMG after day 8 of stimulation. The stimulation protocol of Group B involved a simple increase of the daily rFSH dose to 375 IU after day 8. A total of 40 BMI and age matched patients with an optimal ovarian response formed the control group (Group C).Results: The mean Group A serum concentration of oestradiol on the day of HCG administration and average number of oocytes retrieved were significantly higher than Group B (P < 0.001) and equivalent to Group C. A total of 10 pregnancies (50%) in Group A, 8 (34.8%) in Group B and 19 (47.5%) in the control group were achieved.Conclusions: The data suggest that LH supplementation improves the ovarian outcome in patients characterized by an inadequate initial response to rFSH therapy in a long protocol. [ABSTRACT FROM AUTHOR]

Published
2001
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12. Pregnancy after cytoplasmic transfer in a couple suffering from idiopathic infertility: case report.

Author

Dale, Brian, Wilding, Martin, Botta, Giuseppe, Rasile, Marianna, Marino, Marcella, Di Matteo, Loredana, De Placido, Giuseppe, Izzo, Alfredo, Dale, B, Wilding, M, Botta, G, Rasile, M, Marino, M, Di Matteo, L, De Placido, G, and Izzo, A

Abstract

Couples suffering from idiopathic infertility are among the most difficult to treat in IVF cycles due to the unknown cause of failure to reproduce. This can lead to years of unsuccessful attempts to conceive and often the abandonment of treatment. In some couples, the only observable problem is the development of poor quality embryos. In this case report, we describe the successful use of cytoplasmic donation in a couple where the high level of embryo fragmentation and poor embryo development was thought to be the unique cause of failure to conceive after intracytoplasmic sperm injection (ICSI). We noted a strong reduction in embryo fragmentation and a consequent increase in embryo quality after the treatment. Transfer of four of the embryos receiving donor cytoplasm led to a pregnancy with two gestational sacs and the birth of healthy twins. [ABSTRACT FROM AUTHOR]

Published
2001
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13. Mitochondrial aggregation patterns and activity in human oocytes and preimplantation embryos.

Author

Wilding, Martin, Dale, Brian, Marino, Marcella, di Matteo, Loredana, Alviggi, Carlo, Pisaturo, Maria Laura, Lombardi, Luisa, De Placido, Giuseppe, Wilding, M, Dale, B, Marino, M, di Matteo, L, Alviggi, C, Pisaturo, M L, Lombardi, L, and De Placido, G

Abstract

Mitochondria play a vital role in the metabolism of energy-containing compounds in the oocyte cytoplasm to provide adenosine trisphosphate for fertilization and preimplantation embryo development. In this study, ratiometric confocal microscopy with the mitochondrion-specific membrane potential-sensitive fluorescence dye JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide) was used to measure the activity of mitochondria in human oocytes and developing preimplantation embryos. Mitochondria in oocytes and embryos were characterized by distinct localized aggregation patterns. These patterns however did not determine localized regions of heterogeneity in mitochondrial activity. Mitochondrial activity was analysed during oocyte maturation and after fertilization. The activity of mitochondria in fresh metaphase II oocytes was negatively correlated with maternal age. This trend continued when the activity of developing embryos was analysed. Mitochondrial activity was strongly correlated with the rate of embryo development on day 3 after fertilization, but not on day 2. Partial regression analysis showed that the rate of cleavage of preimplantation embryos was more highly correlated with embryo mitochondrial activity than maternal age. These data suggest that the efficiency of mitochondrial respiration in oocytes and preimplantation embryos is closely correlated with the programmed rate of embryo development, and suggest that maternal age further influences this factor. The loss of mitochondrial activity in oocytes obtained from ageing couples may therefore contribute to lower embryo development and pregnancy rates observed during cycles of IVF. [ABSTRACT FROM AUTHOR]

Published
2001
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14. Endocrinology. Recombinant follicle stimulating hormone is effective in poor responders to highly purified follicle stimulating hormone.

Author

De Placido, G., Alviggi, C., Mollo, A., Strina, I., Varricchio, M.T., and Molis, M.

Abstract

Ovarian stimulation in cases of poor ovarian responsiveness is an important challenge in in-vitro fertilization (IVF) programmes. Despite improvements in oocyte number and quality, an ideal ovarian stimulation strategy has yet to be defined. Here, the results of ovarian stimulation with recombinant follicle stimulating hormone (rFSH) in 28 poor responders to highly purified FSH (FSH-HP) with high basal concentrations of FSH are reported. The protocols used on the FSH-HP and rFSH cycles were identical with the sole exception of the FSH preparation: triptorelin 0.1 mg/day (gonadotrophin-releasing hormone, GnRH-agonist short protocol) and the starting FSH dose of 300 IU/day were administered from day 2 of the menstrual cycle. Ovarian outcome was classified as `normal', `intermediate' and `poor', depending on the number of mature oocytes retrieved and the peak serum oestradiol concentration. Nine of the 28 subjects had an intermediate ovarian response to re-stimulation with rFSH. In the 26 patients who received human chorionic gonadotrophin on both cycles, re-stimulation resulted in a significant increase (P < 0.05) in the mean number of mature oocytes (2.4 ± 1.4 versus 1.7 ± 0.8), mean peak oestradiol concentration (606 ± 252 versus 443 ± 32 pg/ml) and fertilization rate (73.0 versus 53.3%). Four pregnancies were achieved. It is concluded that rFSH in a GnRH-agonist short protocol improves the ovarian outcome in poor responders to FSH-HP with high basal concentrations of FSH. [ABSTRACT FROM PUBLISHER]

Published
2000
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15. Conservative treatment by angiographic uterine artery embolization of a 12 week cervical ectopic pregnancy.

Author

Nappi, C, D'Elia, A, Di Carlo, C, Giordano, E, De Placido, G, and Iaccarino, V

Abstract

A 32 year old woman, gravid 1, nulliparous, was admitted to our department at 11 weeks and 2 days of gestation after being diagnosed with cervical pregnancy. She was unsuccessfully treated with methotrexate for 5 days. On the fifth day after admission she underwent bilateral uterine artery angiographic embolization followed by vacuum evacuation and curettage of the cervical canal. A Foley catheter was also inserted in the cervical canal and left in place for 4 days. The patient was discharged in good condition on the seventh postoperative day. [ABSTRACT FROM AUTHOR]

Published
1999

16. Case report. Conservative treatment by angiographic uterine artery embolization of a 12 week cervical ectopic pregnancy.

Author

Nappi, C, D'Elia, A, Di Carlo, C, Giordano, E, De Placido, G, and Iaccarino, V

Abstract

A 32 year old woman, gravid 1, nulliparous, was admitted to our department at 11 weeks and 2 days of gestation after being diagnosed with cervical pregnancy. She was unsuccessfully treated with methotrexate for 5 days. On the fifth day after admission she underwent bilateral uterine artery angiographic embolization followed by vacuum evacuation and curettage of the cervical canal. A Foley catheter was also inserted in the cervical canal and left in place for 4 days. The patient was discharged in good condition on the seventh postoperative day [ABSTRACT FROM PUBLISHER]

Published
1999
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17. Mitochondrial aggregation patterns and activity in human oocytes and preimplantation embryos

Author

Martin Wilding, Carlo Alviggi, Brian Dale, Maria Laura Pisaturo, Marcella Marino, Loredana Di Matteo, Giuseppe De Placido, Luisa Lombardi, Wilding, M., Dale, B., Marino, M., DI MATTEO, L., Alviggi, Carlo, Pisaturo, M. L., Lombardi, L., DE PLACIDO, Giuseppe, Wilding, M, Dale, B, Marino, M, DI MATTEO, Loredana, Alviggi, C, Pisaturo, Ml, Lombardi, L, DE PLACIDO, G., and DI MATTEO, L

Subjects
animal structures, Cellular respiration, Embryonic Development, Fertilization in Vitro, Mitochondrion, Biology, Cleavage (embryo), Membrane Potentials, Andrology, Human fertilization, Pregnancy, medicine, Humans, Metaphase, Fluorescent Dyes, Genetics, Microscopy, Confocal, Rehabilitation, Embryogenesis, Obstetrics and Gynecology, Embryo, Carbocyanines, Embryo, Mammalian, Oocyte, Mitochondria, medicine.anatomical_structure, Reproductive Medicine, embryonic structures, Oocytes, Regression Analysis, Benzimidazoles, Female, Maternal Age
Abstract

Mitochondria play a vital role in the metabolism of energy-containing compounds in the oocyte cytoplasm to provide adenosine trisphosphate for fertilization and preimplantation embryo development. In this study, ratiometric confocal microscopy with the mitochondrion-specific membrane potential-sensitive fluorescence dye JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide) was used to measure the activity of mitochondria in human oocytes and developing preimplantation embryos. Mitochondria in oocytes and embryos were characterized by distinct localized aggregation patterns. These patterns however did not determine localized regions of heterogeneity in mitochondrial activity. Mitochondrial activity was analysed during oocyte maturation and after fertilization. The activity of mitochondria in fresh metaphase II oocytes was negatively correlated with maternal age. This trend continued when the activity of developing embryos was analysed. Mitochondrial activity was strongly correlated with the rate of embryo development on day 3 after fertilization, but not on day 2. Partial regression analysis showed that the rate of cleavage of preimplantation embryos was more highly correlated with embryo mitochondrial activity than maternal age. These data suggest that the efficiency of mitochondrial respiration in oocytes and preimplantation embryos is closely correlated with the programmed rate of embryo development, and suggest that maternal age further influences this factor. The loss of mitochondrial activity in oocytes obtained from ageing couples may therefore contribute to lower embryo development and pregnancy rates observed during cycles of IVF.

Published
2001
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