1. Effects of different routes of administration on the immunogenicity of the Tat protein and a Tat-derived peptide.
- Author
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Finessi V, Nicoli F, Gallerani E, Sforza F, Sicurella M, Cafaro A, Caputo A, Ensoli B, and Gavioli R
- Subjects
- AIDS Vaccines administration & dosage, Animals, Blood immunology, HIV Antibodies analysis, HIV Antibodies blood, Immunity, Mucosal, Mice, tat Gene Products, Human Immunodeficiency Virus administration & dosage, AIDS Vaccines immunology, Immunity, Humoral, tat Gene Products, Human Immunodeficiency Virus immunology
- Abstract
The use of the Tat protein of HIV in vaccines against AIDS showed promising results in primate and human studies. To characterize the impact of the administration route on the induction of humoral responses at systemic and mucosal levels, we compared intradermal, intramuscular and mucosal immunizations with Tat and a Tat-derived peptide. Mice were immunized with the Tat protein by different routes and the titer and isotype of anti-Tat antibodies were assessed in serum and mucosal lavages. Intramuscular and intradermal administrations showed comparable immunogenicity, while the mucosal administration was unable to induce IgM in serum and IgG at mucosal sites but showed superior immunogenicity in terms of IgA induction. Anti-Tat antibodies were also obtained upon vaccination with the immunodominant Tat 1-20 peptide which was, however, less immunogenic than the whole Tat protein.
- Published
- 2015
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