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Storing biometric templates and/or encryption keys, as adopted in traditional biometrics-based authentication methods, has raised a matter of serious concern. To address such a concern, biometric key generation, which derives encryption keys directly from statistical features of biometric data, has emerged to be a promising approach. Existing methods of this approach, however, are generally unable to appropriately model user variations, making them difficult to produce consistent and discriminative keys of high entropy for authentication purposes. This paper develops a semisupervised clustering scheme, which is optimized through a niching memetic algorithm, to effectively and simultaneously model both intra- and interuser variations. The developed scheme is employed to model the user variations on both single features and feature subsets with the purpose of recovering a large number of consistent and discriminative feature elements for key generation. Moreover, the scheme is designed to output a large number of clusters, thus further assisting in producing long while consistent and discriminative keys. Based on this scheme, a biometric key generation method is finally proposed. The performance of the proposed method has been evaluated on the biometric modality of handwritten signatures and compared with existing methods. The results show that our method can deliver consistent and discriminative keys of high entropy, outperforming-related methods. [ABSTRACT FROM AUTHOR]