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Your search keyword '"Krawczyk, Connie"' showing total 12 results
Start Over Author "Krawczyk, Connie" Journal immunity
12 results on '"Krawczyk, Connie"'

1. Ketolysis drives CD8+ T cell effector function through effects on histone acetylation

Author

Luda, Katarzyna M., Longo, Joseph, Kitchen-Goosen, Susan M., Duimstra, Lauren R., Ma, Eric H., Watson, McLane J., Oswald, Brandon M., Fu, Zhen, Madaj, Zachary, Kupai, Ariana, Dickson, Bradley M., DeCamp, Lisa M., Dahabieh, Michael S., Compton, Shelby E., Teis, Robert, Kaymak, Irem, Lau, Kin H., Kelly, Daniel P., Puchalska, Patrycja, Williams, Kelsey S., Krawczyk, Connie M., Lévesque, Dominique, Boisvert, François-Michel, Sheldon, Ryan D., Rothbart, Scott B., Crawford, Peter A., and Jones, Russell G.

Published
2023
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8. Metabolic Profiling Using Stable Isotope Tracing Reveals Distinct Patterns of Glucose Utilization by Physiologically Activated CD8+ T Cells

Author

Ma, Eric H., primary, Verway, Mark J., additional, Johnson, Radia M., additional, Roy, Dominic G., additional, Steadman, Mya, additional, Hayes, Sebastian, additional, Williams, Kelsey S., additional, Sheldon, Ryan D., additional, Samborska, Bozena, additional, Kosinski, Penelope A., additional, Kim, Hyeryun, additional, Griss, Takla, additional, Faubert, Brandon, additional, Condotta, Stephanie A., additional, Krawczyk, Connie M., additional, DeBerardinis, Ralph J., additional, Stewart, Kelly M., additional, Richer, Martin J., additional, Chubukov, Victor, additional, Roddy, Thomas P., additional, and Jones, Russell G., additional

Published
2019
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11. Metabolic Profiling Using Stable Isotope Tracing Reveals Distinct Patterns of Glucose Utilization by Physiologically Activated CD8 + T Cells.

Author

Ma EH, Verway MJ, Johnson RM, Roy DG, Steadman M, Hayes S, Williams KS, Sheldon RD, Samborska B, Kosinski PA, Kim H, Griss T, Faubert B, Condotta SA, Krawczyk CM, DeBerardinis RJ, Stewart KM, Richer MJ, Chubukov V, Roddy TP, and Jones RG

Subjects
Animals, Cell Proliferation, Gas Chromatography-Mass Spectrometry, Glycolysis, Host-Pathogen Interactions genetics, Host-Pathogen Interactions immunology, Lymphocyte Activation genetics, Mice, Oxidative Stress, Virus Diseases genetics, Virus Diseases immunology, Virus Diseases metabolism, Virus Diseases virology, CD8-Positive T-Lymphocytes immunology, CD8-Positive T-Lymphocytes metabolism, Energy Metabolism, Glucose metabolism, Lymphocyte Activation immunology, Metabolome, Metabolomics methods
Abstract

Naive CD8 + T cells differentiating into effector T cells increase glucose uptake and shift from quiescent to anabolic metabolism. Although much is known about the metabolism of cultured T cells, how T cells use nutrients during immune responses in vivo is less well defined. Here, we combined bioenergetic profiling and 13 C-glucose infusion techniques to investigate the metabolism of CD8 + T cells responding to Listeria infection. In contrast to in vitro-activated T cells, which display hallmarks of Warburg metabolism, physiologically activated CD8 + T cells displayed greater rates of oxidative metabolism, higher bioenergetic capacity, differential use of pyruvate, and prominent flow of 13 C-glucose carbon to anabolic pathways, including nucleotide and serine biosynthesis. Glucose-dependent serine biosynthesis mediated by the enzyme Phgdh was essential for CD8 + T cell expansion in vivo. Our data highlight fundamental differences in glucose use by pathogen-specific T cells in vivo, illustrating the impact of environment on T cell metabolic phenotypes., (Copyright © 2019 Elsevier Inc. All rights reserved.)

Published
2019
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12. Interleukin-10 Directly Inhibits CD8 + T Cell Function by Enhancing N-Glycan Branching to Decrease Antigen Sensitivity.

Author

Smith LK, Boukhaled GM, Condotta SA, Mazouz S, Guthmiller JJ, Vijay R, Butler NS, Bruneau J, Shoukry NH, Krawczyk CM, and Richer MJ

Subjects
Animals, Antigens, Viral immunology, Female, Galectins physiology, Glycosylation, Lymphocytic choriomeningitis virus immunology, Male, Mice, Mice, Inbred C57BL, N-Acetylglucosaminyltransferases physiology, Receptors, Antigen, T-Cell physiology, Signal Transduction physiology, CD8-Positive T-Lymphocytes immunology, Interleukin-10 physiology
Abstract

Chronic viral infections remain a global health concern. The early events that facilitate viral persistence have been linked to the activity of the immunoregulatory cytokine IL-10. However, the mechanisms by which IL-10 facilitates the establishment of chronic infection are not fully understood. Herein, we demonstrated that the antigen sensitivity of CD8 + T cells was decreased during chronic infection and that this was directly mediated by IL-10. Mechanistically, we showed that IL-10 induced the expression of Mgat5, a glycosyltransferase that enhances N-glycan branching on surface glycoproteins. Increased N-glycan branching on CD8 + T cells promoted the formation of a galectin 3-mediated membrane lattice, which restricted the interaction of key glycoproteins, ultimately increasing the antigenic threshold required for T cell activation. Our study identified a regulatory loop in which IL-10 directly restricts CD8 + T cell activation and function through modification of cell surface glycosylation allowing the establishment of chronic infection., (Copyright © 2018 Elsevier Inc. All rights reserved.)

Published
2018
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