1. Real-Time Manipulation of T Cell-Dendritic Cell Interactions In Vivo Reveals the Importance of Prolonged Contacts for CD4+ T Cell Activation
- Author
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Fabrice Lemaître, Philippe Bousso, Susanna Celli, Dynamiques des Réponses Immunes, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), This work is supported by Pasteur Institute, Inserm, Mairie de Paris, Fondation de France, and by a Marie Curie Excellence grant., We wish to thank E. Robey, M. Albert, and J. Di Santo for helpful comments on the manuscript, J,-J. Deschamps and G. Massonneau for help with intravital setup, and Ana-Maria Lennon-Duménil and Danielle Lankar for the Y3P Ab., Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Celli, Susanna
- Subjects
CD4-Positive T-Lymphocytes ,Time Factors ,MESH: Flow Cytometry ,Cell Communication ,Lymphocyte Activation ,Mice ,0302 clinical medicine ,Immunology and Allergy ,MESH: Animals ,Cells, Cultured ,Antigen Presentation ,Microscopy ,0303 health sciences ,MESH: Dendritic Cells ,biology ,Cd4 t cell ,medicine.diagnostic_test ,MESH: CD4-Positive T-Lymphocytes ,CD28 ,Flow Cytometry ,Cell biology ,Infectious Diseases ,medicine.anatomical_structure ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,MESH: Cells, Cultured ,MESH: Microscopy ,[SDV.IMM] Life Sciences [q-bio]/Immunology ,T cell ,Immunology ,Major histocompatibility complex ,Flow cytometry ,03 medical and health sciences ,Antigen ,MESH: Mice, Inbred C57BL ,In vivo ,MESH: Cell Communication ,medicine ,Animals ,MESH: Lymphocyte Activation ,MESH: Mice ,030304 developmental biology ,MESH: Time Factors ,Dendritic Cells ,Dendritic cell ,Mice, Inbred C57BL ,CELLIMMUNO ,MESH: Antigen Presentation ,Immunologic Techniques ,biology.protein ,MESH: Immunologic Techniques ,030215 immunology - Abstract
International audience; T cells interact with dendritic cells (DCs) for periods lasting from minutes to hours. However, a causal link between the duration of this interaction and the efficiency of T cell activation has not been established in vivo. Employing intravital two-photon imaging, we manipulated T cell-DC interactions in real time and found that the first T cell-DC encounter often resulted in a long-lived interaction. Moreover, the cessation of T cell receptor-major histocompatibility complex signals promoted cellular dissociation, suggesting that antigen availability on DCs regulates contact duration. Finally, at least 6 hr of in vivo T cell-DC interaction were required for naive CD4 + T cells to undergo clonal expansion. These results establish the importance of prolonged T cell-DC interactions for efficient CD4 + T cell activation in vivo.
- Published
- 2007
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