1. CD161 expression defines new human γδ T cell subsets.
- Author
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Karunathilaka, Amali, Halstrom, Samuel, Price, Patricia, Holt, Michael, Lutzky, Viviana P., Doolan, Denise L., Kupz, Andreas, Bell, Scott C., Thomson, Rachel M., Miles, John J., and Ratnatunga, Champa N.
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T cells , *SELF-organizing maps , *OLDER people , *PSYCHONEUROIMMUNOLOGY , *FLOW cytometry , *CD8 antigen - Abstract
γδ T cells are a highly versatile immune lineage involved in host defense and homeostasis, but questions remain around their heterogeneity, precise function and role during health and disease. We used multi−parametric flow cytometry, dimensionality reduction, unsupervised clustering, and self-organizing maps (SOM) to identify novel γδ T cell naïve/memory subsets chiefly defined by CD161 expression levels, a surface membrane receptor that can be activating or suppressive. We used middle-to-old age individuals given immune blockade is commonly used in this population. Whilst most Vδ1+subset cells exhibited a terminal differentiation phenotype, Vδ1− subset cells showed an early memory phenotype. Dimensionality reduction revealed eight γδ T cell clusters chiefly diverging through CD161 expression with CD4 and CD8 expression limited to specific subpopulations. Comparison of matched healthy elderly individuals to bronchiectasis patients revealed elevated Vδ1+ terminally differentiated effector memory cells in patients potentially linking this population with chronic proinflammatory disease. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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