1. Reprint of: MicroRNA profiling of human intermediate monocytes
- Author
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Kyrill S. Rogacev, Adam M. Zawada, Danilo Fliser, Lu Zhang, Björn Rotter, Yvan Devaux, Loems Ziegler-Heitbrock, Nicolas Krezdorn, Gunnar H. Heine, and Insa E. Emrich
- Subjects
Risk ,0301 basic medicine ,Cellular differentiation ,CD14 ,Immunology ,Population ,Lipopolysaccharide Receptors ,Context (language use) ,Biology ,CD16 ,Monocytes ,Epigenesis, Genetic ,03 medical and health sciences ,microRNA ,medicine ,Humans ,Immunology and Allergy ,education ,Cells, Cultured ,Regulation of gene expression ,education.field_of_study ,Genome ,Sequence Analysis, RNA ,Gene Expression Profiling ,Monocyte ,Receptors, IgG ,Cell Differentiation ,Hematology ,Cell biology ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Cardiovascular Diseases - Abstract
Among the three human monocyte subsets, intermediate CD14++CD16+ monocytes have been characterized as particularly proinflammatory cells in experimental studies and as potential biomarkers of cardiovascular risk in clinical cohorts. To further substantiate the distinct role of intermediate monocytes within human monocyte heterogeneity, we assessed subset-specific expression of miRNAs as central epigenetic regulators of gene expression. We hypothesized that intermediate monocytes have a distinct miRNA profile compared to classical and non-classical monocytes. By using small RNA-seq we analyzed 662 miRNAs in the three monocyte subsets. We identified 38 miRNAs that are differentially expressed in intermediate monocytes compared to both classical and non-classical monocytes with a p value of
- Published
- 2017