1. The function of the octamer-binding site in the DRA promoter
- Author
-
Nabila Jabrane-Ferrat, B. Matija Peterlin, and Charles F. Voliva
- Subjects
Transcription, Genetic ,TATA box ,Immunology ,Molecular Sequence Data ,DNA, Recombinant ,HLA-DR alpha-Chains ,Biology ,Regulatory Sequences, Nucleic Acid ,Genetics ,Tumor Cells, Cultured ,Humans ,Histone octamer ,Binding site ,Adenovirus E1B Proteins ,Promoter Regions, Genetic ,Binding Sites ,Base Sequence ,TATA-Box Binding Protein ,Promoter ,HLA-DR Antigens ,Molecular biology ,Burkitt Lymphoma ,TATA Box ,Class II gene ,DNA-Binding Proteins ,Transcription Factor TFIID ,biology.protein ,TATA-binding protein ,Octamer Transcription Factor-2 ,HeLa Cells ,Transcription Factors - Abstract
The octamer binding site, which is located immediately upstream of the poorly conserved DRA TATA sequence, is important for high levels of expression of this human major histocompatibility class II gene in B cells. In this study, we demonstrate that the substitution of the DRA TATA sequence with the TATA box from the adenovirus E1b promoter removes the requirement for the octamer binding site for high levels of expression from the DRA promoter. Since only the TATA box from the E1b but not the DRA promoters binds the TATA binding protein, we conclude that the octamer binding site helps to recruit TBP to the DRA promoter.
- Published
- 1996