1. Trimethyltin reduces ATP levels and MTT reduction in the LRM55 rat astrocytoma cell line.
- Author
-
Eyer CL, Rio C, and Smith JR
- Subjects
- Animals, Astrocytoma enzymology, Astrocytoma pathology, Coloring Agents metabolism, L-Lactate Dehydrogenase metabolism, Oxidation-Reduction drug effects, Rats, Tumor Cells, Cultured, Adenosine Triphosphate antagonists & inhibitors, Adenosine Triphosphate metabolism, Astrocytoma metabolism, Tetrazolium Salts metabolism, Thiazoles antagonists & inhibitors, Thiazoles metabolism, Trimethyltin Compounds pharmacology
- Abstract
The LRM55 rat astrocytoma cell line was used to study the time and concentration effects of trimethyltin (TMT) exposure on intracellular adenosine triphosphate (ATP) levels, formazan production from (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), and lactate dehydrogenase (LDH) release. TMT concentrations of > or =50 microM produced a delayed increase in extracellular LDH from approximately 20% at 24 h to almost 70%, at 72 h. Twenty-four hours before cell lysis was detectable ATP levels decreased to less than 30% and formazan production declined to 70% (50 microM), 31% (100 microM), and 21% (200 microM) of control values. Concentrations of TMT (5 and 10 microM) that produced little or no LDH release also decreased ATP levels (62 and 49% of control, respectively) and formazan production (63 and 52% of control, respectively) by 48 h. These data support the hypothesis that TMT exposure interferes with energy production and that this event likely contributes to the delayed cell death seen in this cell line. Moreover, the declines in ATP and formazan production can occur at subtoxic concentrations in LRM55 cells.
- Published
- 2000