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The aim of the present research was to perform green synthesis of gold nanoparticles of isolated citrus bioflavonoid from Citrus sinensis (orange) peel extract and to evaluate its anticancer potential. Methanolic extract of orange peel was obtained by Soxhlet extraction and citrus bioflavonoid was isolated by using column chromatography. Gold nanoparticles were developed by green synthesis method, wherein 1 % aqueous solution of isolated citrus bioflavonoid were mixed with 1% solution of HAuCl4 and incubated at ambient temperature for 4 to 5 hours and observed for the color change which indicated formation of nanoparticles. Obtained gold nanoparticles were evaluated by UV visible spectra, FTIR, SEM, XRD analysis and for antimicrobial potential against E coli, S.aureus and P. aeruginosa. Cytotoxicity study was carried out by using in vitro assays, namely MTT, SRB and Tryphan blue assay, against colon cancer cell line Colo 320 DM, and HT 29. results of SEM showed that nanosized particles in the range of 80-100nm were formed. Results of cytotoxicity studies revealed that CBFGNP exhibited 72.28% inhibition, against Colo320 DM whereas pure CBF showed 70.46% inhibition. Against HT 29, CBFGNP exhibited 69.79% inhibition, whereas for MTT assay and SRB assay, CBFGNP showed 80.15% and 58.29% inhibition, respectively. Moreover, CBFGNP exhibited 90.29% and 85% non viability against Colo320 DM and HT29. Based on the results, it can be concluded that gold nanoparticles of citrus bioflavonoid (CBFGNP) exhibits more cytotoxicity against Colo320 DM and HT29 as compared to pure citrus bioflavonoid when assessed by three different in vitro cytotoxicity assays.

In the recent years, the interests on the herbal medicines and their utilization for the treatment for various diseases have been increasing rapidly, even in those areas where modern medicine is available. Medicinal plants are the richest source of bioactive compounds used in the traditional and modern medicine. Nowadays, demand for the plant based medicines, health products, pharmaceuticals, food supplements has been increasing due to their effectiveness. The objective of the present study was to formulate and evaluate herbal cough syrup from the petal extracts of Calendula officinalis L. The syrup formulation was designed by using petal extract, honey and required amount of distilled water. The prepared herbal cough syrup was evaluated for different parameters such as pH, color, odor, taste, thermal stability, turbidity, accelerated stability studies, HPTLC and antimicrobial activity against different microbial strains namely Aspergillus niger, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus and E. coli. HPTLC analysis of the prepared formulations was compared with standard marker compound namely Quercetin. The Rf of Quercetin perfectly matched with the spot observed in Formulation A and B. From the results of the study, it can be concluded that the prepared syrup formulation comprising of aqueous extract of C. officinalis exhibited good antimicrobial activity against the selected microbial strains, and it exhibited soothing effect with sweet taste because of honey.

The present work was aimed to formulate various cosmetic formulations comprising of extract of Piper betel and Acacia catechu and limestone as a coloring agent to minimize the side effect(s) associated with the use of synthetic colorants. Different natural ingredients such as bees wax, carnauba wax, white soft paraffin, castor oil, strawberry essence, rice starch and lemon juice were used to formulate herbal lipstick, rouge, and eye shadow along with the extracted color. Different evaluation tests were carried out to evaluate the prepared formulations such as color, texture, pH, perfume stability, melting point, breaking point, softening point, surface anomalies, aging stability, thixotrophy and skin irritation test. The results of the evaluation tests revealed that melting point of the prepared lipstick formulation was in the range of 61-63oC. The breaking point of all the prepared lipstick formulations was in the range of 30 – 33 g. Force of application was noted to be good for all the formulations. Thixotrophy character was observed in the range of 9.2 ±0.3 to 9.6 ±0.1. Softening point was found to be in the range 52.33 to 60.330C. Eye shadow and rouge showed good adhesion property and proper color dispersion with melting point of 480C. Patch test on the volunteers showed no signs of skin irritation. The results of the study confirmed that the color extracted from Piper betel and Acacia catechu may prove to be an attractive option and act as a substitute for synthetic colorants in various cosmetic formulations.

The present investigation was undertaken to assess the hypoglycemic potential of Caesalpinia bonducella (C.bonducella) and Myristica fragrans (M.fragrans), employing various in vitro techniques. The extracts of seeds of C. bonducella and M. fragrans were studied for their effects on glucose adsorption capacity, in vitro glucose diffusion, in vitro amylolysis kinetics and glucose transport across the yeast cells. It was observed that the plant extracts under study adsorbed glucose and the adsorption of glucose increased remarkably with an increase in glucose concentration. There were no significant (p≤0.05) differences between their adsorption capacities. The results of amylolysis kinetic experimental model revealed that the rate of glucose diffusion was found to be increased with time from 30 to 180 min and both the plant extracts demonstrated significant inhibitory effects on movement of glucose into external solution across dialysis membrane as compared to control. Also, the plant extracts promoted glucose uptake by the yeast cells. It was observed that the enhancement of glucose uptake was dependent on both the sample and glucose concentration. C. bonducella extract exhibited significantly higher (p≤0.05) activity than the extract of M. fragrans at all concentrations. The results of the study verified the hypoglycemic activity of the extracts of C. bonducella and M. fragrans. However, the observed effects exhibited by the extracts of seeds of C. bonducella and M. fragrans need to be confirmed by using different in vivo models and clinical trials for their effective utilization as therapeutic agents in better management of diabetes mellitus.