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Isolation of intact, double stranded, pure and non- contaminated genomic DNA is prerequisite for large scale genotyping analysis including DNA-banks. Three methods of DNA isolation (Dellaporta, CTAB and Hi-PurAg DNA isolation kits) from 25 black gram genotypes were compared in terms of the yield, purity, integrity, and stability of extracted DNA. Purity and quantification of isolated DNA samples was confirmed by using the UV nano-spectrophotometer at OD260/280 and the same is confirmed based by agarose gel electrophoresis. The CTAB method showed the best results followed by Hi- PurAg and Dellaporta method. The CTAB DNA extraction method was found to be the most efficient DNA extraction method, capable of providing high quality, pure and stable DNA and could be used for various molecular related works. All the 25 black gram genotypes for this research gave good yield of DNA from the established modified CTAB protocol. [ABSTRACT FROM AUTHOR]

Sesame is notable for its rich antioxidants and sesame oil is also familiar for its resistance to oxidation. The cultivation of sesame is usually restricted under abiotic stress conditions. Hence in order to study the performance of sesame genotypes to saline stress, an experiment was conducted to screen ten ruling varieties of sesame viz., CO1, SVR1, SVPR1, VRI 1, VRI 2, TMV 3, TMV 4, TMV 5, TMV 6 and TMV 7 at five different EC levels (EC 1, 2, 3, 4 and 5). Seedling observations namely, germination percentage, speed of germination, shoot length, root length and dry matter production were recorded. Among the genotypes, it was found that under higher saline conditions (EC5), VRI 1 recorded higher seedling length (2.0 cm), vigour index I (100) and vigour index II (1.15), whereas under normal condition, TMV 4 and CO 1 performed better. In general, there was a decrease in all the seedling parameters with increased levels of salinity. With STAR software, Multivariate analysis was done and on the basis of dendrogram, five clusters were formed and the cophenotic correlation co- efficient was found highest in Control (0.912) followed by EC 2 ( 0.829). [ABSTRACT FROM AUTHOR]

Investigations were carried out to study the influence of flyash seed pelleting on microbial populations of bacteria, fungi, actinomycetes, and rhizobium in black gram and green gram. The experimental results revealed that seed pelleting with fly ash seed pelleting @ 250 g with rice gruel as adhesive increased rhizobium population in black gram and fungi and actinomyctes population in green gram by 10 to 15%. Increased microbial activity will enrich soil microflora and in turn will be helpful in increasing the crop yield. [ABSTRACT FROM AUTHOR]

Pulses are one of the most important legume crops in India contributing significantly high quality protein. Among the legumes, black gram (Vigna mungo L. Hepper), is an important legume crop in our country belonging to the family leguminoceae. The low productivity in pulses may be due to the fact that they are normally grown in marginal lands with inadequate soil moisture and poor fertility status. To increase yield under these circumstances, integrated seed treatment is given as a presowing treatment. Hence, a study was attempted to find out the influence of integrated seed treatments on growth and yield parameters in black gram. The seeds of black gram cv VBN 4 were subjected to various seed treatments and treated seeds along with the control were evaluated for their seed quality, growth, gas exchange and yield parameters both in laboratory and field trials. The study revealed that seeds fortified with MgSO4 + polykote + carbendazim + dimethoate + bioinoculant (Pseudomononas fluorescens) + Rhizobium + Azophos + pelleting with DAP recorded higher seed yield and other parameters when compared to control. [ABSTRACT FROM AUTHOR]

Submergence tolerance is an important character to be considered when flash flood damages rice. Tolerant genotypes can withstand submergence for 1-2 weeks based on their tolerance level. Hence with a view to study the effect of submergence on germination and seedling attributes, eight cultivated varieties were subjected to submergence tolerance at five levels of flooding viz., 1 cm, 2 cm, 3 cm, 4 cm and 5 cm flooding levels. CR 1009 sub 1, a submergence tolerant variety was used as check. With increase in submergence levels, greater reduction was observed for all the parameters. Survival % and seedling length were found to be decreased under flooded conditions, but to a much lower extent in the tolerant genotype. Flooding decreased shoot, root and total dry matter production in all the varieties with more reduction in higher flooding (5 cm) rather than lower flooding (1cm) levels. [ABSTRACT FROM AUTHOR]

Laboratory experiments were conducted to study the effect of different pre storage treatments and packaging materials on storability of Jatropha seeds. The treatments included halogen mixture @ 3g kg-1, Thiram @ 2g kg-1 and packed in three different containers viz., Aluminium container, Polythene bag and Cloth bag. The seeds were evaluated at monthly intervals upto ten months and observations on germination percent and vigour index were recorded in two varieties viz., Chidambaram local and Sirkali local. The results revealed that Jatropha seeds treated with halogen mixture and stored in aluminium container could be stored upto 7 months without loss in vigour and viability. [ABSTRACT FROM AUTHOR]

Field experiments were conducted to study the effect of fly ash seed pelleting in rice under aerobic agriculture which can be recommended for better crop growth and establishment. The study was conducted with three concentrations viz., 200, 250 and 300g per kg of seed. Observations on growth and yield parameters viz., plant height, dry matter production, leaf area, root weight, number of productive tillers/plant, number of grains/panicle, 1000 grain weight, and single plant yield were recorded. Gas exchange parameters viz., net photosynthetic rate, leaf transpiration rate, stomatal conductance and intercellular CO2 concentration were also recorded. The experimental results revealed that aerobic rice seeds treated with fly ash @ 250 g kg-1 performed better due to increased growth, gas exchange parameters and seed yield. [ABSTRACT FROM AUTHOR]

Field experiments were conducted to study the effect of seed pelleting with flyash at different concentrations ranging from 200 to 300g per kg of seed on growth, gas exchange parameters and seed yield in sesame. Observations on growth and yield parameters viz., plant height, number of branches, number of leaves, leaf area, dry matter production, root length, root weight, number of capsules, number of seeds per capsule, 1000 seed weight, seed yield per plant and gas exchange parameters viz., net photosynthetic rate, leaf temperature, stomatal conductance and intercellular CO2 were recorded. Rhizosphere population of bacteria, fungi, actinomycetes and azospirillum were also estimated. Based on the results, it is found that sesame seeds treated with flyash @ 250 g,kg-1with rice gruel recorded the increased seed yield (Trial I - 11.867 g/plant and Trial II - 12.31 g/ plant) due to increase in growth and gas exchange parameters. [ABSTRACT FROM AUTHOR]

The present study was undertaken to explore the characters highly responsible for yield of tomato. Genotypic variability, heritability and genetic advance for yield and yield contributing characters were studied for 18 tomato genotypes and data were recorded on plant height at maturity, number of branches per plant, days to 50 percent flowering, number of fruits per cluster, number of fruits per plant, average fruit weight, number of locule per fruit and fruit yield per plant. The experimental results revealed that the phenotypic variation was high as compared to genotypic variation for all the traits studied and maximum for yield per plant, average fruit weight and plant height. High PCV, GCV and genetic advance was observed for yield per plant, number of fruits per plant and number of locules per fruit indicating the additive genetic effect. Phenotypic selection for their improvement could be achieved by simple selection. [ABSTRACT FROM AUTHOR]

Black gram [Vigna mungo (L.) Hepper] is the third most important pulse crop in India after chickpea and pigeon pea. Soil salinity is one of the major factor responsible for loss in agricultural production. Forty eight black gram genotypes were evaluated for salt tolerance with six different salinity levels viz., EC0, EC4, EC7, EC10, EC13, EC16 at early seedling stage. All the seedling parameters and salt tolerant index were investigated from four days old seedlings. From the results, it was found that there was a gradual decrease with increasing levels of salinity in all the genotypes studied. Seed germination and dry matter production showed more variations than other parameters studied for all the genotypes at different salinity levels. Based on the results, it may be concluded that the genotypes VNBG 017, AUB 3 AND AUB 20 were saline tolerant and VNBG 022, AUB 31 AND AUB 12 were susceptible to salinity. [ABSTRACT FROM AUTHOR]

Here an efficient protocol for micropropagation of black gram [Vigna mungo (L.) Hepper] cv. VBN 3 is reported. The deembryonated cotyledonary explants were cultured on MS medium containing different concentrations of plant growth regulators. The maximum frequency (72%) of direct shoot regeneration (devoid of callus phase), multiple shoot induction and shoot elongation was achieved from culturing the explants on MS medium containing 3.0 mg/l of 6-benzylaminopurine (BAP). Up to 65% of the regenerated shoots were rooted on MS medium containing 0.25 mg/l of a-naphthalene acetic acid (NAA) within 3 weeks after subculturing. The in vitro-raised plantlets were successfully hardened first under culture room conditions with 62% survival rate and then in greenhouse. The identified regeneration system could be efficiently used in various in vitro manipulation studies in black gram as well. [ABSTRACT FROM AUTHOR]