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Autism is a developmental disorder characterized by troubles with social interaction and communication by restricted and repetitive behavior.The parents usually notice signs in the first two or three years of their child's life. These signs often develop gradually. Blood samples were taken for 60 people with autism and 30 healthy people as a control group. Both groups ranged age from 3 to 8 years . After completion of number, required tests were carried out on samples. The tests were three biochemical markers. The first is neurotransmitters (γABA), the other enzymes of happiness (Serotonin & Melatonin), all this biochemical markers were measured by elisa technique, the results were a decrease in level of γABA for ASD as compare with control group, A significant increases in serotonin in ASD. relative to control group, and no change in level of Melatonin in both ASD and healthy group these results consistent with many research and another research inconsistent with these results have shown the reasons that led to differences in results. [ABSTRACT FROM AUTHOR]