1. Characterization of an Australian isolate of taro bacilliform virus and development of an infectious clone
- Author
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Dawit B. Kidanemariam, Grahame V. H. Jackson, Amit C. Sukal, Kathy Crew, Anthony P. James, Adane Abraham, Robert M. Harding, and James L. Dale
- Subjects
0106 biological sciences ,0301 basic medicine ,clone (Java method) ,060506 Virology ,Genome, Viral ,Biology ,01 natural sciences ,Genome ,Polymerase Chain Reaction ,Virus ,law.invention ,03 medical and health sciences ,Open Reading Frames ,law ,Virology ,Plant virus ,Polymerase chain reaction ,Phylogeny ,Plant Diseases ,Australia ,infectious clone ,General Medicine ,Sequence Analysis, DNA ,badnavirus ,Clone Cells ,Colocasia esculenta ,Badnavirus ,030104 developmental biology ,taro ,010606 plant biology & botany ,Taro bacilliform virus ,Colocasia - Abstract
The badnavirus taro bacilliform virus (TaBV) has been reported to infect taro (Colocasia esculenta L.) and other edible aroids in several South Pacific island countries, but there are no published reports from Australia. Using PCR and RCA, we identified and characterized an Australian TaBV isolate. A terminally redundant cloned copy of the TaBV genome was generated and shown to be infectious in taro following agro-inoculation. This is the first report of TaBV from Australia and also the first report of an infectious clone for this virus.
- Published
- 2018