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A novel, green and efficient in-situ cloud point-reinforced ionic liquid-assisted mechanochemical extraction (IS-CPR-IL-MCE) technique coupled with ultrahigh performance liquid chromatography was established to simultaneously extract, concentrate and separate five alkaloids (including liensinine, isoliensinine, neferine, O-demethyl nuciferine and nuciferine) from lotus leaf. The parameters affecting the extraction efficiency of target analytes, such as the amount of IL, surfactant and salt, grinding time, pH, enrichment temperature and time were systematically studied and optimized by single-factor and multifactor experiments. The highest extraction yield of alkaloids was obtained by IS-CPR-IL-MCE under the following conditions: 0.23 g of 1-dodecyl-3-methylimidazolium bromide, 0.37 g of Triton X-100, 7.73 of pH, 5 min of milling time, 2.5 g of (NH 4) 2 SO 4 , 60 ℃ and 20 min of enrichment temperature and time. The developed method exhibited a good linearity in the range of 0.5–100.0 μg/mL with the coefficients of correction more than 0.9992, low limit of detection of 8.0–11.2 ng/mL, acceptable reproducibility below 4.45%, and satisfactory recovery in the range of 80.4–93.7% The results demonstrated that the proposed method was successfully applied to the extraction of alkaloids from lotus leaf. • An ionic liquid assisted mechanochemical extraction was developed. • This method was reinforced by in-situ cloud point extraction. • This method was suitable for simultaneous extraction and enrichment of alkaloids. • Environmental protection, efficient extraction and good repeatability were achieved. [ABSTRACT FROM AUTHOR]

• An in situ effervescence reaction-assisted MCAE method is proposed. • Ganoderma lucidum samples are mechanically ground with solid acid-base reagents. • Ganoderic acids are extracted by water instead of an organic solvent. • The approach has the advantages of simple operation and high efficiency. The aim of this work was to develop a simple, green and low-consumption method for the extraction of ganoderic acids. An in situ effervescence reaction-assisted mechanochemical extraction method was proposed, and the effects of independent variations in terms of sodium bicarbonate concentration, milling time, and liquid/solid ratio (mL/g) were investigated by means of a response surface methodology (RSM) Box-Behnken design (BBD). The highest yields of ganoderic acids were achieved by grinding a Ganoderma lucidum sample with sodium bicarbonate (372.60 mg/g) and citric acid for 0.87 min with a water to material ratio of 10.58 mL. The linear calibration ranges were from 0.01–5 μg/mL for the six analytes and had good linearity with a correlation coefficient of determination higher than 0.9991. In addition, the obtained LODs of the six target analytes were 1.46 − 2.97 ng/mL, and the LOQs calculated as ten times the signal-to-noise ratio were 4.88 − 9.90 ng/mL. The proposed method was successfully applied to the Ganoderma lucidum samples. [ABSTRACT FROM AUTHOR]