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Highlights: [•] We reviewed the molecular approaches used to characterize dermatophyte species. [•] Dermatophytes taxonomy changed based on phylogenetic analyses of the ITS region. [•] Micro-satellite and sequence analyses of the NTS region of rDNA typed dermatophytes. [•] Microsporum and Trichophyton species have a recent radiation. [ABSTRACT FROM AUTHOR]

Abstract: Porcine parvovirus (PPV) is widespread among swine and is responsible for reproductive failure of susceptible sows, characterized by embryonic and fetal death. Studies showed that PPV in domestic pig is genetically diverse and some strains differ from the ones used for vaccination. Organ samples from wild boars and domestic pigs were collected in Transylvania (Romania) and tested for the presence of PPV by polymerase chain reaction. Positive samples were grouped and 14 from the wild boar and 1 from the domestic pig PPVs were selected for VP1/VP2 sequence analysis and comparison with available GenBank data. The molecular clock analysis revealed that PPV has a relatively recent evolutionary history, originated approximately 120years ago and the main divergence occurred in the last 20–60years. Phylogenetic and residue substitution analysis showed that the viruses could be divided into 6 distinct clusters and that wild boar PPVs were partially different and independent from domestic pig PPVs. PPVs of wild boars proved to be more diverse than viruses of domestic pigs. The presence of the highly virulent 27a-like PPV strains in wild boars was also detected. [Copyright &y& Elsevier]

Entamoeba histolytica is a protozoan parasite and the causative agent of amoebiasis in humans. The estimations of the worldwide burden of amoebiasis by the WHO indicated that approximately 500 million people were infected with the parasite and 10% of these individuals had invasive amoebiasis. However, our understanding of the disease burden and epidemiology of human amebiasis has undergone dramatic changes over the last two decades based on molecular analyses. The development of Entamoeba genomics has also provided some interesting and valuable information on the evolution and population structure of this parasite. In addition, the use of a number of molecular markers has greatly expanded our understanding of Entamoeba host range and genetic diversity. In this review, we re-assessed Entamoeba prevalence and species in humans, non-human primates, other animals, and the environment in the context of molecular data. Some issues regarding the evolution and phylogeny of different Entamoeba species lineages are also discussed. Unlabelled Image • The global impact of amebic infection remains substantial based on the molecular data; • More molecular studies are needed to further expand our understanding of Entamoeba genetic diversity; • The genetic structure of Entamoeba populations and disease-related genes are needed to be explored. [ABSTRACT FROM AUTHOR]