1. Novel herpesviruses in neotropical bats and their relationship with other members of the Herpesviridae family
- Author
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Damien Donato, Anne Lavergne, Benoit de Thoisy, Vincent Lacoste, Samantha James, Laboratoire des Interactions Virus-Hôtes [Cayenne, Guyane Française], Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Ecole Doctorale 587 : Diversités, santé et développement en Amazonie (ED 587), Université de Guyane (UG), Département de Virologie - Department of Virology, Institut Pasteur [Paris] (IP), Biologie des Infections Virales Émergentes - Biology of Emerging Viral Infections (UBIVE), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] (IP), This study benefited from the CAROLIA and RESERVOIRS programs supported by European funds (ERDF/FEDER) and assistance from Région Guyane and Direction Régionale pour la Recherche et la Technologie. It also received a European Commission 'REGPOT-CT-2011-285837-STRonGer' grant within the FP7 and 'Investissement d'Avenir' grants managed by the Agence Nationale de la Recherche (CEBA, Ref. ANR-10-LABX-25-01). S.J. was supported by a grant from the Université de la Guyane, Ecole doctorale 587 'Diversités, Santé et Développement en Amazonie' and by a grant from the Collectivité Territoriale de la Guyane., All field volunteers and owners and/or managers of capture sites are warmly acknowledged for their assistance in captures., ANR-10-LABX-0025,CEBA,CEnter of the study of Biodiversity in Amazonia(2010), European Project: 285837,EC:FP7:REGPOT,FP7-REGPOT-2011-1,STRONGER(2011), Lacoste, Vincent, Laboratoires d'excellence - CEnter of the study of Biodiversity in Amazonia - - CEBA2010 - ANR-10-LABX-0025 - LABX - VALID, Strengthening transdisciplinary research on infectious and emerging diseases in French Guiana: linking fieldwork, benchside and bedside - STRONGER - - EC:FP7:REGPOT2011-11-01 - 2015-04-30 - 285837 - VALID, Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] (IP), Institut Pasteur [Paris], and Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris]
- Subjects
0301 basic medicine ,[SDV]Life Sciences [q-bio] ,DNA-Directed DNA Polymerase ,[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy ,Mormoopidae ,law.invention ,law ,Chiroptera ,Gammaherpesvirinae ,Molossidae ,Herpesviridae ,Phylogeny ,Polymerase chain reaction ,[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Carollia perspicillata ,biology ,Phylogenetic tree ,[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] ,Betaherpesvirus ,French Guiana ,3. Good health ,[SDV] Life Sciences [q-bio] ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Infectious Diseases ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Glycoprotein B ,Microbiology (medical) ,Evolution ,030106 microbiology ,[SDV.BID]Life Sciences [q-bio]/Biodiversity ,DNA polymerase ,Microbiology ,Article ,Gammaherpesvirus ,Viral Proteins ,03 medical and health sciences ,Phylogenetics ,[SDV.BID.SPT] Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy ,[SDV.BID.EVO] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] ,Genetics ,Animals ,Martinique ,[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,Glycoproteins ,biology.organism_classification ,Exodeoxyribonucleases ,030104 developmental biology ,Evolutionary biology ,Desmodus rotundus ,[SDV.BID] Life Sciences [q-bio]/Biodiversity - Abstract
In the past decade, a large number of studies have detected herpesvirus sequences from many bat species around the world. Nevertheless, the discovery of bat herpesviruses is geographically uneven. Of the various bat species tested to date, only a few were from the New World. Seeking to investigate the distribution and diversity of herpesviruses circulating in neotropical bats, we carried out molecular screening of 195 blood DNA samples from 11 species of three bat families (Phyllostomidae, Mormoopidae, and Molossidae). Using polymerase chain reaction amplification, with degenerate consensus primers targeting highly conserved amino acid motifs of the herpesvirus DNA polymerase and Glycoprotein B genes, we characterized novel viral sequences from all tested species. BLAST searches, pairwise nucleotide and amino acid sequence comparisons, as well as phylogenetic analyses confirmed that they all belonged to the Herpesviridae family, of the Beta- and Gammaherpesvirinae subfamilies. Fourteen partial DNA polymerase gene sequences, of which three beta- and 11 gamma-herpesviruses, were detected. A total of 12 partial Glycoprotein B gene sequences, all gamma-herpesviruses, were characterized. Every sequence was specific to a bat species and in some species (Desmodus rotundus, Carollia perspicillata, and Pteronotus rubiginosus) multiple viruses were found. Phylogenetic analyses of beta- and gammaherpesvirus sequences led to the identification of bat-specific clades. Those composed of sequences obtained from different bat species belonging to distinct subfamilies follow the taxonomy of bats. This study confirms the astonishing diversity of bat herpesviruses and broadens our knowledge of their host range. Nevertheless, it also emphasizes the fact that, to better appreciate the evolutionary history of these viruses, much remains to be done at various taxonomic levels., Highlights • Molecular screening was carried out on 11 bat species from French Guiana and Martinique (French West Indies). • Partial DNA polymerase gene sequences of 14 viruses were characterized, three beta- and 11 gammaherpesviruses, as well as 12 Glycoprotein B sequences, all of gamma-herpesviruses. • Genetic characterization of these sequences reveals a high degree of genetic divergence. • On a phylogenetic viewpoint, most of the newly discovered herpesvirus sequences fall within bat-specific clades well correlated with the taxonomy of their hosts. • This study is the largest conducted to date in terms of species diversity from the New World.
- Published
- 2020
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