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Although at least 30 novel hantaviruses have been recently discovered in novel hosts such as shrews, moles and even bats, hantaviruses (family Bunyaviridae , genus Hantavirus) are primarily known as rodent-borne human pathogens. Here we report on identification of a novel hantavirus variant associated with a rodent host, Major’s pine vole ( Microtus majori ). Altogether 36 hantavirus PCR-positive Major’s pine voles were identified in the Krasnodar region of southern European Russia within the years 2008–2011. Initial partial L-segment sequence analysis revealed novel hantavirus sequences. Moreover, we found a single common vole ( Microtus arvalis ) infected with Tula virus (TULV). Complete S- and M-segment coding sequences were determined from 11 Major’s pine voles originating from 8 trapping sites and subjected to phylogenetic analyses. The data obtained show that Major’s pine vole is a newly recognized hantavirus reservoir host. The newfound virus, provisionally called Adler hantavirus (ADLV), is closely related to TULV. Based on amino acid differences to TULV (5.6–8.2% for nucleocapsid protein, 9.4–9.5% for glycoprotein precursor) we propose to consider ADLV as a genotype of TULV. Occurrence of ADLV and TULV in the same region suggests that ADLV is not only a geographical variant of TULV but a host-specific genotype. High intra-cluster nucleotide sequence variability (up to 18%) and geographic clustering indicate long-term presence of the virus in this region.