1. Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in community setting in Casablanca
- Author
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Abouddihaj Barguigua, Naima El Mdaghri, Rachid Saile, Fatima El Otmani, Mohammed El Azhari, Mohammed Timinouni, and Hind Ouair
- Subjects
Microbiology (medical) ,Adult ,Male ,Serratia ,Klebsiella pneumoniae ,Microbial Sensitivity Tests ,medicine.disease_cause ,beta-Lactam Resistance ,beta-Lactamases ,Microbiology ,Feces ,Young Adult ,Ciprofloxacin ,Enterobacter cloacae ,polycyclic compounds ,medicine ,Humans ,Escherichia coli ,Aged ,General Immunology and Microbiology ,biology ,Enterobacteriaceae Infections ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,Middle Aged ,bacterial infections and mycoses ,biology.organism_classification ,Enterobacteriaceae ,Anti-Bacterial Agents ,Morocco ,Infectious Diseases ,Carriage ,Carrier State ,bacteria ,Community setting ,Female ,Fecal carriage ,Norfloxacin - Abstract
The importance of community-acquired infections due to extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) has been increasingly recognized in recent years. This study aimed to determine the prevalence of intestinal carriage of ESBL-PE in the community in Casablanca, Morocco.During 6 months (2013), 93 fecal samples were examined for ESBL-PE. Isolates expressing an ESBL phenotype were investigated for the presence of genes encoding β-lactamases and plasmid-mediated quinolone resistance. Conjugation experiments were done to determine the mobility of ESBL genes.The prevalence of fecal carriage of ESBL-PE was 4.3% (4/93; 95% CI, 0.2-8.4). Klebsiella pneumoniae (n = 2), Enterobacter cloacae (n = 2), Escherichia coli (n = 1), and Serratia odorifera (n = 1) were the ESBL-producing species. Four (66.7%) of these isolates were multidrug-resistant. The blaSHV-12 (n = 5) was the most frequent ESBL gene detected, followed by blaCTX-M-15 (n = 3).The non-ESBL gene detected was blaTEM-1 (n = 5). One isolate harbored the qnrB1 variant. RESULTS of conjugation experiments indicated that blaSHV-12 + blaTEM-1 + qnrB1 and blaCTX-M-15 + blaTEM-1 genes were co-transferred and that these genes were carried by a conjugative plasmid of high molecular weight (125 kb).Our results show the importance of the intestinal tract as a reservoir for ESBL-PE in the community in Morocco.
- Published
- 2014