1. Geniposide inhibits interleukin-6 and interleukin-8 production in lipopolysaccharide-induced human umbilical vein endothelial cells by blocking p38 and ERK1/2 signaling pathways
- Author
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Wen-Ming Li, Ying-Xiong Wang, Yuguang Du, Chao Yu, Hongtao Liu, Juan Yin, Jun-Lin He, and Zhu Yang
- Subjects
Lipopolysaccharides ,MAPK/ERK pathway ,Umbilical Veins ,Chemokine ,Cell Survival ,p38 mitogen-activated protein kinases ,Blotting, Western ,Immunology ,Enzyme-Linked Immunosorbent Assay ,p38 Mitogen-Activated Protein Kinases ,Monocytes ,Umbilical vein ,Cell Movement ,Cell Adhesion ,Humans ,Iridoids ,Interleukin 8 ,Extracellular Signal-Regulated MAP Kinases ,Cell adhesion ,Pharmacology ,Wound Healing ,U937 cell ,biology ,Interleukin-6 ,Reverse Transcriptase Polymerase Chain Reaction ,Interleukin-8 ,NF-kappa B ,Endothelial Cells ,U937 Cells ,Molecular biology ,embryonic structures ,cardiovascular system ,biology.protein ,I-kappa B Proteins ,Indicators and Reagents ,Signal transduction ,Signal Transduction - Abstract
The aim of this study was to investigate the inhibitory effect of geniposide on lipopolysaccharide (LPS)-induced interleukin-6 (IL-6) and interleukin-8 (IL-8) production in human umbilical vein endothelial cells (HUVECs).Primary HUVECs were used. The mRNA/protein levels of IL-6 and IL-8 was determined by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). LPS-induced HUVEC migration and adhesion of monocytes to HUVECs were studied by monolayer wound healing experiments and monocytic cell adhesion assay, respectively. Expression of nuclear factor kappaB (NF-kappaB), inhibitory factor kappaB-alpha (IkappaB-alpha), p38 mitogen-activated protein kinase (MAPK) and ERK1/2 were determined by Western blot analysis.Geniposide effectively inhibited LPS-induced expression of IL-6 and IL-8 in HUVECs at the transcription and translation levels. Additionally, geniposide suppressed LPS-induced HUVEC migration and U937 monocyte adhesion to HUVECs. Signal transduction studies indicate that geniposide blocked the activation of NF-kappaB, degradation of IkappaB-alpha, and phosphorylation of p38 MAPK and ERK1/2 in HUVECs challenged by LPS.The results show that geniposide can inhibit LPS-induced IL-6 and IL-8 production in HUVECs by blocking p38 MAPK and ERK1/2 signaling pathways.
- Published
- 2009