1. Influence of insulin on the microvascular response to inflammatory mediators in neonatal streptozotocin diabetic rats.
- Author
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Rastelli VM, Akamine EH, Oliveira MA, Nigro D, Passaglia Rde C, Carvalho MH, and Fortes ZB
- Subjects
- Animals, Animals, Newborn, Blood Glucose metabolism, Blood Pressure, Diabetes Mellitus, Experimental blood, Diabetes Mellitus, Experimental chemically induced, Insulin blood, Male, Platelet Activating Factor pharmacology, Rats, Rats, Wistar, Streptozocin pharmacology, Diabetes Mellitus, Experimental physiopathology, Inflammation Mediators pharmacology, Insulin pharmacology, Microcirculation drug effects
- Abstract
Objectives: To investigate the effect of insulin on microvascular responses to inflammatory mediators in a model of type 2 diabetes mellitus., Materials: We used the neonatal streptozotocin (n-STZ)-induced diabetes model. Diabetes was induced in male newborn (2-day-old) Wistar rats through STZ administration. Experiments were performed 10-12 weeks later., Methods: Rats were divided into control (sham-injected) and study (n-STZ) groups. Using a closed-circuit video camera coupled to a microscope, changes in mesenteric arteriolar and venular diameters induced by topical application of the inflammatory mediators histamine, bradykinin and platelet-activating factor were assessed in chloral hydrate-anesthetized rats., Treatment: The n-STZ rats received NPH insulin s.c. for either 4 h or 12 days., Results: Impaired arteriole and venule responses to the inflammatory mediators tested were observed in n-STZ rats. Both acute and chronic insulin treatment corrected the alterations., Conclusion: We conclude that insulin is beneficial, restoring microvascular reactivity to inflammatory mediators in type 2 diabetes.
- Published
- 2005
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