1. A new copper(II) selective fluorescence probe based on naphthalimide: Synthesis, mechanism and application in living cells.
- Author
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He, Guangjie, Meng, Qingtao, Zhao, Xiuwen, He, Cheng, Zhou, Peng, and Duan, Chunying
- Subjects
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COMPLEX compounds synthesis , *COPPER compounds , *NAPHTHALIMIDES , *METAL compounds , *FLUORESCENT probes , *REACTION mechanisms (Chemistry) - Abstract
A new fluorescence probe L based on naphthalimide has been synthesized for selective and quantitative detection of Cu 2 + in CH 3 CN:H 2 O (4:1, v / v ) solution. L exhibited a strong green fluorescence. Upon addition of 2 equiv. of Cu 2 + , the fluorescence emission shows a steady and smooth decrease until a plateau is reached with a 30-fold quenching of fluorescence intensity. In the presence of Cu 2 + , the absorbance peak of L maximum at 466 nm decreased, and a new absorption band at 600 nm appeared. Under the identical conditions, other physiological and environmental important metal ions induced negligible spectroscopic changes. The 1:2 stoichiometry binding mode of L with Cu 2 + was supported by the Benesi–Hildebrand analysis and ESI-MS spectra studies. The detection limit for Cu 2 + was estimated to be 64 ppb . Fluorescence microscopy experiments showed that L has practical application in living cells. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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