1. Mandibular glands secrete 24-methylenecholesterol into honey bee (Apis mellifera) food jelly.
- Author
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Buttstedt, Anja, Pirk, Christian W.W., and Yusuf, Abdullahi A.
- Subjects
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HONEYBEES , *GLANDS , *ROYAL jelly , *JELLY , *FOOD production , *HONEY , *HYPOPHARYNX , *SUBMANDIBULAR gland - Abstract
Honey bee (Apis mellifera) workers feed their larvae with food jelly that is secreted by specialized glands in their heads – the hypopharyngeal and the mandibular glands. Food jelly contains all the nutrients the larvae need to develop into adult honey bees, including essential dietary sterols. The main sterol in food jelly, 24-methylenecholesterol (24MC), is pollen-derived and delivered in food jelly to the larvae in a complex with two proteins, major royal jelly protein 1 (MRJP1) and apisim. Whereas the proteins are synthesized in the hypopharyngeal glands, the sterol-secreting gland has not been identified. We here identified the mandibular glands as sterol-secreting gland for food jelly production by direct detection of the four main honey bee sterols (24MC, campesterol, β-sitosterol and isofucosterol). Furthermore, 24MC seems to be specifically enriched in the mandibular glands, thereby ensuring that food jelly contains the amounts of 24MC necessary for complex formation with MRJP1 and apisimin. [Display omitted] • We here identified the mandibular glands as source of sterols in food jelly. • 24-methylenecholesterol seems to be specifically enriched in mandibular glands. • The fatty acid 10-HDA is not only detected in high amounts in mandibular glands of nurse bees, but also in foragers. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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