1. Association of TNF-α gene variations with thoracic aortic dissection risk in a Chinese Han population.
- Author
-
DU XM, Liu LW, DU ZK, Gu RX, Han YL, and Wang XZ
- Subjects
- Adult, Aortic Dissection diagnosis, Aortic Dissection ethnology, Aortic Aneurysm, Thoracic blood, Aortic Aneurysm, Thoracic diagnosis, Aortic Aneurysm, Thoracic ethnology, Asian People genetics, Case-Control Studies, China epidemiology, Disease Progression, Female, Gene Frequency, Genetic Association Studies, Genetic Predisposition to Disease, Humans, Male, Middle Aged, Phenotype, Promoter Regions, Genetic, RNA, Messenger blood, RNA, Messenger genetics, Risk Factors, Tumor Necrosis Factor-alpha blood, Aortic Dissection genetics, Aortic Aneurysm, Thoracic genetics, Tumor Necrosis Factor-alpha genetics
- Abstract
Background: Chronic inflammation may be involved in pathogenesis of thoracic aortic dissection (TAD). Tumor necrosis factor-alpha (TNF-α) is a proinflammatory cytokine that plays an important role in pathological TAD progression. In this study, we determined wether genetic variants of TNF-α were associated with TAD., Methods: Frequency distributions of TNF-α promoter polymorphisms (-1031C/T,-857C/T,-308G/A, and -238G/A) were determined by direct sequencing. TNF-α plasma levels were measured by enzyme-linked immunosorbent assay. Plasma levels of TNF-α mRNA in peripheral-blood mononuclear cells were analyzed by real-time quantitative polymerase chain reaction amplification., Results: We found the TNF-α promoter -857C/T polymorphism is associated with disease progression susceptibility in TAD patients. The CC homozygote of TAD patients had a significantly higher risk of TAD than did T allele carriers (P< 0.05). Plasma TNF-α concentrations were also significantly higher in TAD patients than control subjects (P<0.05), and CC genotype carriers showed increased TNF-α levels compared with T allele carriers (P<0.05). Moreover, peripheral-blood mononuclear cells carrying the CC genotype showed increased TNF-α mRNA levels compared with cells carrying the T allele., Conclusions: The -857C/T polymorphism of TNF-α promoter plays a role in the genetic variation underlying susceptibility of individuals to TAD progression. The CC genotype is associated with increased TNF-α expression in TAD patients, and may be an independent predictive factor for TAD.
- Published
- 2016