1. Analysis of Innate and Adaptive Immunological Characteristics in Patients with IgG4-Related Disease.
- Author
-
Sohn, Kyoung-Hee, Ham, Jongho, Chung, Soo Jie, Kang, Hye-Ryun, and Kim, Hye Young
- Subjects
T helper cells ,INNATE lymphoid cells ,CELL populations ,B cells ,T cells - Abstract
Background: Immunoglobulin G4-related disease (IgG4-RD) is a systemic immunological disorder characterized by fibro-inflammatory conditions; however, the pathobiology of IgG4-RD has not been fully identified. Objective: This study aimed to analyze systemic differences of innate and adaptive immune cells from healthy controls and patients with IgG4-RD. Methods: Healthy controls (n = 9) and IgG4-RD patients (n = 7) were recruited with informed consent. Peripheral blood was collected from healthy controls and IgG4-RD patients, and three blood samples from IgG4-RD patients were re-collected two months after the last rituximab (RTX) treatment. The various immune cells and cytokine productions were measured by flow cytometry. Results: Blood CD14
+ monocytes and steady-state follicular helper T cells were increased in patients with IgG4-RD. However, there were no changes in other immune cell populations, including B cells, CD4 T cells, CD8 T cells, and innate lymphoid cells. Also, the TGF-β-producing CD14+ monocytes were significantly augmented in patients with IgG4-RD. Two months after RTX treatment, total B cells (CD19+ ) were depleted; however, the expressions of TGF-β from CD14+ monocytes remained unchanged. Conclusion: These findings showed that IgG4-RD is related to the increment of CD14+ monocytes. Besides, controlling increased TGF-β-producing CD14+ monocytes with RTX treatment might be a conducive way to regulate IgG4-RD. [ABSTRACT FROM AUTHOR]- Published
- 2020
- Full Text
- View/download PDF