1. Oral vaccination with invasive Lactobacillus plantarum delivered nucleic acid vaccine co-expressing SS1 and murine interleukin-4 elicits protective immunity against Trichinella spiralis in BALB/c mice.
- Author
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Xue Y, Yang KD, Quan Y, Jiang YL, Wang N, Huang HB, Lu HN, Zhu ZY, Zhang B, Li JY, Pan TX, Shi CW, Yang GL, and Wang CF
- Subjects
- Administration, Oral, Animals, Blotting, Western, Endodeoxyribonucleases immunology, Fluorescent Antibody Technique, Helminth Proteins immunology, Interleukin-4 immunology, Male, Mice, Mice, Inbred BALB C, Trichinellosis immunology, Vaccines, Synthetic therapeutic use, Bacterial Vaccines therapeutic use, Endodeoxyribonucleases genetics, Helminth Proteins genetics, Interleukin-4 genetics, Lactobacillus plantarum immunology, Nucleic Acid-Based Vaccines therapeutic use, Trichinella spiralis immunology, Trichinellosis prevention & control
- Abstract
Trichinellosis is a foodborne zoonosis caused by Trichinella spiralis (T. spiralis) that not only causes considerable economic losses for the global pig breeding and food industries, but also seriously threats the health of human. Therefore, it is very necessary to develop an effective vaccine to prevent trichinellosis. In this study, the invasive Lactobacillus plantarum (L. plantarum) expressing fibronectin-binding protein A (FnBPA) was served as a live bacterial vector to deliver DNA to the host to produce a novel oral DNA vaccine. Co-expressing T. spiralis SS1 and murine interleukin-4 (mIL-4) of DNA vaccine were constructed and subsequently delivered to intestinal epithelial cells via invasive L. plantarum. At 10 days after the third immunization, the experimental mice were challenged with 350 T. spiralis infective larvae. The results found that the mice orally vaccinated with invasive L. plantarum harboring pValac-SS1/pSIP409-FnBPA not only stimulated the production of anti-SS1-specific IgG, Th1/Th2 cell cytokines, and secreted(s) IgA but also decreased worm burden and intestinal damage. However, the mice inoculated with invasive L. plantarum co-expressing SS1 and mIL-4 (pValac-SS1-IL-4/pSIP409-FnBPA) induced the highest protective immune response against T. spiralis infection. The DNA vaccine delivered by invasive L. plantarum provides a novel idea for the prevention of T. spiralis infection., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
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