1. Modulation of expression of IL-8 gene in bronchial epithelial cells by 5-methoxypsoralen
- Author
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Nicolis, Elena, Lampronti, Ilaria, Dechecchi, Maria Cristina, Borgatti, Monica, Tamanini, Anna, Bezzerri, Valentino, Bianchi, Nicoletta, Mazzon, Martina, Mancini, Irene, Giri, Maria Grazia, Rizzotti, Paolo, Gambari, Roberto, and Cabrini, Giulio
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GENETIC regulation , *INTERLEUKIN-8 , *BRONCHI , *EPITHELIAL cells , *PSORALENS , *NEUTROPHILS , *CYSTIC fibrosis , *RESPIRATORY diseases , *PSEUDOMONAS aeruginosa , *PATIENTS ,RESPIRATORY organ microbiology - Abstract
Abstract: Persistent recruitment of neutrophils in the bronchi of cystic fibrosis patients contributes to airway tissue damage, suggesting the importance of intervening on the expression of the neutrophil chemokine IL-8. Extracts from plants have been investigated to select components able to reduce IL-8 expression in bronchial epithelial cells challenged with Pseudomonas aeruginosa. Extracts and purified components have been added to cells 24h before pro-inflammatory challenge with P. aeruginosa and IL-8 transcription was quantified in the IB3-1 CF cells in vitro. P. aeruginosa-dependent IL-8 mRNA induction was increased by Argemone mexicana and Vernonia anthelmintica whereas no significant modification of transcription was observed with Aphanamixis polystachya, Lagerstroemia speciosa and Hemidesmus indicus. Finally, inhibition of IL-8 was observed with Polyalthia longifolia (IC50 =200μg/ml) and Aegle marmelos (IC50 =20μg/ml). Compounds from A. marmelos were isolated and identified by GC–MS. No significant effect was observed with butyl-p-tolyl sulphate, whereas the inhibition obtained with 6-methyl-4-chromanone concentration was accompanied by an anti-proliferative effect. On the contrary, 5-methoxypsoralen resulted in IL-8 inhibition at 10μM concentration, without effects on cell proliferation. In synthesis, 5-methoxypsoralen can be taken into consideration to investigate mechanisms of neutrophil chemotactic signalling and for its potential application in modulating the excessive CF lung inflammation. [Copyright &y& Elsevier]
- Published
- 2009
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