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Start Over Author "Ran, Jihua" Journal international immunopharmacology
3 results on '"Ran, Jihua"'

2. Geniposide suppresses LPS-induced nitric oxide, PGE2 and inflammatory cytokine by downregulating NF-κB, MAPK and AP-1 signaling pathways in macrophages

Author

Xinchuan Zheng, Liu Zhengxiang, Li Fang, Li Xiaoling, Shi Qinghai, Jinjun Cao, Hongming Zhang, Zhao Hongyan, Ying Ran, Yu Zhou, Fu Jianfeng, Ran Jihua, Ge Di, and Wang Li

Subjects
MAPK/ERK pathway, Lipopolysaccharides, Male, medicine.medical_specialty, Lipopolysaccharide, Pyridines, medicine.medical_treatment, Immunology, Anti-Inflammatory Agents, Nitric Oxide Synthase Type II, Inflammation, Mice, Inbred Strains, Biology, Pharmacology, Nitric Oxide, Dinoprostone, Nitric oxide, Cell Line, chemistry.chemical_compound, Mice, Internal medicine, Nitriles, medicine, Butadienes, Immunology and Allergy, Animals, Iridoids, Sulfones, Extracellular Signal-Regulated MAP Kinases, Interleukin-6, Tumor Necrosis Factor-alpha, Imidazoles, NF-kappa B, NF-κB, Gardenia, Nitric oxide synthase, Transcription Factor AP-1, Endocrinology, Cytokine, chemistry, Cyclooxygenase 2, biology.protein, Macrophages, Peritoneal, Tumor necrosis factor alpha, medicine.symptom, Inflammation Mediators, Signal Transduction
Abstract

Inflammatory responses are important to host immune reactions, but uncontrolled inflammatory mediators may aid in the pathogenesis of other inflammatory diseases. Geniposide, an iridoid glycoside found in the herb gardenia, is believed to have broad-spectrum anti-inflammatory effects in murine models but its mechanism of action is unclear. We investigated the action of this compound in murine macrophages stimulated by lipopolysaccharide (LPS), as the stimulation of macrophages by LPS is known to induce inflammatory reactions. We determined the effect of geniposide on LPS-induced production of the inflammatory mediators, nitric oxide (NO) and prostaglandin E2 (PGE2), the mRNA and protein expression of the NO and PGE2 synthases, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), respectively, and the mRNA and protein expression of the inflammatory cytokine, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Furthermore, nuclear factor (NF)-κB, mitogen-activated protein kinase (MAPK) and activator protein (AP)-1 activity were assayed. To understand the action of geniposide on the NF-κB and MAPK pathways, we studied the effect of NF-κB and MAPK inhibitors on the LPS-induced production of NO, PGE2 and TNF-α. Our findings clearly showed that geniposide mainly exerts its anti-inflammatory effects by inhibiting the LPS-induced NF-κB, MAPK and AP-1 signaling pathways in macrophages, which subsequently reduces overexpression of the inducible enzymes iNOS and COX-2 and suppresses the expression and release of the inflammatory factors, TNF-α, IL-6, NO and PGE2. Thus, geniposide shows promise as a therapeutic agent in inflammatory diseases.

Published
2013

3. Geniposide suppresses LPS-induced nitric oxide, PGE2 and inflammatory cytokine by downregulating NF-κB, MAPK and AP-1 signaling pathways in macrophages.

Author

Shi, Qinghai, Cao, Jinjun, Fang, Li, Zhao, Hongyan, Liu, Zhengxiang, Ran, Jihua, Zheng, Xinchuan, Li, Xiaoling, Zhou, Yu, Ge, Di, Zhang, Hongming, Wang, Li, Ran, Ying, and Fu, Jianfeng

Subjects
*IRIDOIDS, *PHYSIOLOGICAL effects of lipopolysaccharides, *PROSTAGLANDINS E, *PHYSIOLOGICAL effects of nitric oxide, *MITOGEN-activated protein kinase regulation, *TRANSCRIPTION factors, *CYTOKINES, *CELLULAR signal transduction, *MACROPHAGES
Abstract

Abstract: Inflammatory responses are important to host immune reactions, but uncontrolled inflammatory mediators may aid in the pathogenesis of other inflammatory diseases. Geniposide, an iridoid glycoside found in the herb gardenia, is believed to have broad-spectrum anti-inflammatory effects in murine models but its mechanism of action is unclear. We investigated the action of this compound in murine macrophages stimulated by lipopolysaccharide (LPS), as the stimulation of macrophages by LPS is known to induce inflammatory reactions. We determined the effect of geniposide on LPS-induced production of the inflammatory mediators, nitric oxide (NO) and prostaglandin E2 (PGE2), the mRNA and protein expression of the NO and PGE2 synthases, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), respectively, and the mRNA and protein expression of the inflammatory cytokine, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Furthermore, nuclear factor (NF)-κB, mitogen-activated protein kinase (MAPK) and activator protein (AP)-1 activity were assayed. To understand the action of geniposide on the NF-κB and MAPK pathways, we studied the effect of NF-κB and MAPK inhibitors on the LPS-induced production of NO, PGE2 and TNF-α. Our findings clearly showed that geniposide mainly exerts its anti-inflammatory effects by inhibiting the LPS-induced NF-κB, MAPK and AP-1 signaling pathways in macrophages, which subsequently reduces overexpression of the inducible enzymes iNOS and COX-2 and suppresses the expression and release of the inflammatory factors, TNF-α, IL-6, NO and PGE2. Thus, geniposide shows promise as a therapeutic agent in inflammatory diseases. [Copyright &y& Elsevier]

Published
2014
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