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The facility layout design problem is an extensively studied research problem and belongs to nonpolynomial hard (NP-hard) combinatorial optimization problem. Quadratic assignment problem (QAP) is one of the formulations that is investigated for facility layout design because of its wide applicability. Ant colony optimization (ACO), a biologically inspired heuristic has centered on solving the QAP by achieving approximation as good as possible. This paper presents a population-based hybrid ant system (PHAS), which is an extension of the hybrid ant system (HAS) in which the size of the ant colony has been fixed. The performance of the proposed ant algorithm for QAP is compared with the existing metaheuristic implementations such as tabu search, reactive tabu search, simulated annealing, genetic hybrid method, HAS, and max–min ant system. The experimental results show that the proposed PHAS perform significantly better than the other existing algorithms of QAP. [ABSTRACT FROM AUTHOR]