1. Diversity in Seed Storage Proteins in Maize Genetic Resources: I. Variation in Alcohol Soluble Zein Protein Fraction.
- Author
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Khan, Aamir Hamid, Khan, Nadar, Minhas, Nasir Muhammad, Rabbani, Malik Ashiq, and Ghafoor, Abdul
- Subjects
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POLYPEPTIDES , *ZEIN (Plant protein) , *POLYACRYLAMIDE gel electrophoresis , *MOLECULAR size , *GERMPLASM , *BIOACCUMULATION - Abstract
Being predominant protein of maize seed, its nutritional quality is dependent on composition of zein polypeptides. The major storage protein of maize seed is alcohol soluble zein, which accounts for more than 50% of total seed storage protein. The variation of zein fraction of seed storage protein in maize genetic resources was assessed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Variation in terms of absence and presence, intensity and molecular size was observed in zein polypeptides. On the basis of variation in banding patterns of zein polypeptides, the maize genetic resources were classified into five variation groups which highlight the wide variation in zein polypeptides among maize genetic resources. The group 2 exhibited high accumulation of 70 and 60 kDa zein polypeptides and group 3 exhibited high accumulation of 25 and 20 kDa zein polypeptides as compared to group 1, 4 and 5.These maize genetic resources might have significant implications for nutritional quality improvement of maize.© 2014 Friends Science Publishers [ABSTRACT FROM AUTHOR]
- Published
- 2014